Droplet-interface-bilayer assays in microfluidic passive networks

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Basic biophysical studies and pharmacological processes can be investigated by mimicking the intracellular and extracellular environments across an artificial cell membrane construct. The ability to reproduce in vitro simplified scenarios found in live cell membranes in an automated manner has great potential for a variety of synthetic biology and compound screening applications. Here, we present a fully integrated microfluidic system for the production of artificial lipid bilayers based on the miniaturisation of droplet-interface-bilayer (DIB) techniques. The platform uses a microfluidic design that enables the controlled positioning and storage of phospholipid-stabilized water-in-oil droplets, leading successfully to the scalable and automated formation of arrays of DIBs to mimic cell membrane processes. To ensure robustness of operation, we have investigated how lipid concentration, immiscible phase flow velocities and the device geometrical parameters affect the system performance. Finally, we produced proof-of-concept data showing that diffusive transport of molecules and ions across on-chip DIBs can be studied and quantified using fluorescence-based assays.
LanguageEnglish
Article number9951
Pages1-8
Number of pages8
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 24 Apr 2015

Fingerprint

Microfluidics
Cell Membrane
Biophysical Phenomena
Synthetic Biology
Artificial Cells
Miniaturization
Artificial Membranes
Ion Transport
Lipid Bilayers
Phospholipids
Oils
Fluorescence
Lipids
Equipment and Supplies
Water

Keywords

  • pharmacological science
  • extracellular space
  • intracellular metabolites
  • cell membrane
  • in vitro
  • synthetic biology
  • microfluidic systems
  • artificial lipid bilayers
  • phospholipid
  • water-in-oil microemulsions
  • arrays
  • DIBs
  • lipid content

Cite this

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title = "Droplet-interface-bilayer assays in microfluidic passive networks",
abstract = "Basic biophysical studies and pharmacological processes can be investigated by mimicking the intracellular and extracellular environments across an artificial cell membrane construct. The ability to reproduce in vitro simplified scenarios found in live cell membranes in an automated manner has great potential for a variety of synthetic biology and compound screening applications. Here, we present a fully integrated microfluidic system for the production of artificial lipid bilayers based on the miniaturisation of droplet-interface-bilayer (DIB) techniques. The platform uses a microfluidic design that enables the controlled positioning and storage of phospholipid-stabilized water-in-oil droplets, leading successfully to the scalable and automated formation of arrays of DIBs to mimic cell membrane processes. To ensure robustness of operation, we have investigated how lipid concentration, immiscible phase flow velocities and the device geometrical parameters affect the system performance. Finally, we produced proof-of-concept data showing that diffusive transport of molecules and ions across on-chip DIBs can be studied and quantified using fluorescence-based assays.",
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Droplet-interface-bilayer assays in microfluidic passive networks. / Schlicht, Bárbara; Zagnoni, Michele.

In: Scientific Reports, Vol. 5, 9951, 24.04.2015, p. 1-8.

Research output: Contribution to journalArticle

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AU - Zagnoni, Michele

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