DPN writing on non-flat gold surfaces and detection by SERS

Robert J. Stokes, Jennifer A. Dougan, Eleanore Irvine, Jenifer Ohayon, Sergy Rozhok, Tom Levesque, Bruce Dudzik, Mike Nelson, Duncan Graham

Research output: Contribution to journalConference articlepeer-review


There is a need for fast, reliable and sensitive biosensor arrays. We have used nanostructured plasmonic gold surfaces for the detection of biological species by surface enhanced resonance Raman scattering (SERRS). Careful, directed placement by Dip-Pen Nanolithography (DPN) of the biological species or capture chemistry, within the array facilitates efficient read out via fast Raman line mapping. In addition, we can apply parallel deposition methods to enhance the throughput of these combined techniques. SERRS is an extremely sensitive spectroscopic technique that offers several advantages over conventional fluorescence detection. For example, the high sensitivity of the method allows detection of DNA capture from single plasmonic array "pixels" ∼1 μm2 in area. Additionally, the information rich nature of the SERRS spectrum allows multiple levels of detection to be embedded into each pixel, further increasing the information depth of the array. By moving from micro-to nano-scale features, sensor chips can contain up to 105 times more information, dramatically increasing the capacity for disease screening.

Original languageEnglish
Article number720703
Number of pages7
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 5 May 2009
EventMicrofluidics, BioMEMS, and Medical Microsystems VII - San Jose, CA, United States
Duration: 26 Jan 200928 Jan 2009


  • DNA
  • DPN
  • non-flat gold surfaces
  • biosensor arrays
  • nanostructured plasmonic gold surfaces


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