Does Litomosoides sigmodontis synthesize dimethylethanolamine from choline?

K.M. Houston, S.A. Babayan, J.E. Allen, W. Harnett

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4 Citations (Scopus)

Abstract

Juvenile female Litomosoides sigmodontis secrete a protein (Juv-p120) highly modified with dimethylethanolamine (DMAE). In an attempt to establish the source of this decoration worms were pulsed with [3H]-choline and [3H]-ethanolamine and the radio-isotope labelled products analysed. Both isotope labels were successfully taken up by the worms, as demonstrated by labelling of phospholipids with [3H]-choline, being predominantly incorporated into phosphatidylcholine and [3H]-ethanolamine into phosphatidylethanolamine. Isotope labelling of phosphatidylethanolamine was particularly striking with the worms taking up ~30 times as much labelled ethanolamine as choline. It was possible to detect faint labelling of Juv-p120 with [3H]-ethanolamine after prolonged exposure periods but, unlike the situation with the phospholipids, it was much more readily labelled with [3H]-choline. When pulsing with [3H]-ethanolamine it was also possible to detect isotope-labelled phosphatidylcholine, which may ultimately account for the low levels of labelling of Juv-p120. Overall our results raise the previously unconsidered but intriguing possibility that in L. sigmodontis, choline may be the precursor of DMAE.
Original languageEnglish
Pages (from-to)55-61
Number of pages6
JournalParasitology
Volume135
Issue number1
DOIs
Publication statusPublished - 2009

Keywords

  • choline
  • dimethylethanolamine
  • filarial nematode
  • Litomosoides
  • phospholipid

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