DNA sequence recognition by an imidazole-containing isopropyl thiazole polyamide (thiazotropsin b)

C.J. Suckling, Andrew J. Hampshire, Hannah Khairallah, A.I. Khalaf, Abdolrasoul H. Ebrahimabadi, R.D. Waigh, Tom Brown, Keith R. Fox

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have used DNA footprinting and fluorescence melting experiments to study the sequence specific binding of an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) to DNA. While the parent compound (thiazotropsin A) binds to the hexanucleotide sequence ACTAGT, changing one of the N-methylpyrrole groups to N-methylimidazole changes the preferred binding sequence to (A/T)CGCG(T/A). Experiments with DNA fragments that contain variants of this sequence suggest that the ligand can also bind, with lower affinity, to sequences which differ from this by 1 bp in any position.

LanguageEnglish
Pages3469-3474
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Volume16
Issue number13
DOIs
Publication statusPublished - 1 Jul 2006

Fingerprint

Thiazoles
DNA sequences
Nylons
DNA Footprinting
DNA
Freezing
Fluorescence
Ligands
Melting
Experiments
imidazole
1-methylimidazole
thiazotropsin A
thiazotropsin B
N-methylpyrrole

Keywords

  • DNA recognition
  • minor groove ligand
  • footprinting

Cite this

Suckling, C. J., Hampshire, A. J., Khairallah, H., Khalaf, A. I., Ebrahimabadi, A. H., Waigh, R. D., ... Fox, K. R. (2006). DNA sequence recognition by an imidazole-containing isopropyl thiazole polyamide (thiazotropsin b). Bioorganic and Medicinal Chemistry Letters, 16(13), 3469-3474. https://doi.org/10.1016/j.bmcl.2006.04.007
Suckling, C.J. ; Hampshire, Andrew J. ; Khairallah, Hannah ; Khalaf, A.I. ; Ebrahimabadi, Abdolrasoul H. ; Waigh, R.D. ; Brown, Tom ; Fox, Keith R. / DNA sequence recognition by an imidazole-containing isopropyl thiazole polyamide (thiazotropsin b). In: Bioorganic and Medicinal Chemistry Letters. 2006 ; Vol. 16, No. 13. pp. 3469-3474.
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Suckling, CJ, Hampshire, AJ, Khairallah, H, Khalaf, AI, Ebrahimabadi, AH, Waigh, RD, Brown, T & Fox, KR 2006, 'DNA sequence recognition by an imidazole-containing isopropyl thiazole polyamide (thiazotropsin b)' Bioorganic and Medicinal Chemistry Letters, vol. 16, no. 13, pp. 3469-3474. https://doi.org/10.1016/j.bmcl.2006.04.007

DNA sequence recognition by an imidazole-containing isopropyl thiazole polyamide (thiazotropsin b). / Suckling, C.J.; Hampshire, Andrew J.; Khairallah, Hannah; Khalaf, A.I.; Ebrahimabadi, Abdolrasoul H.; Waigh, R.D.; Brown, Tom; Fox, Keith R.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 16, No. 13, 01.07.2006, p. 3469-3474.

Research output: Contribution to journalArticle

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T1 - DNA sequence recognition by an imidazole-containing isopropyl thiazole polyamide (thiazotropsin b)

AU - Suckling, C.J.

AU - Hampshire, Andrew J.

AU - Khairallah, Hannah

AU - Khalaf, A.I.

AU - Ebrahimabadi, Abdolrasoul H.

AU - Waigh, R.D.

AU - Brown, Tom

AU - Fox, Keith R.

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KW - DNA recognition

KW - minor groove ligand

KW - footprinting

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