DNA profiling of shahtoosh

A.M.T. Linacre, J.C. Lee, L. Tsai, C. Yang, H. Hsieh, H. Chiang, S. Lai, N. Huang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The population of the Tibetan Antelope (Pantholops hodgsonii) has recently declined dramatically due to the illegal trade in its wool. The animal lives at high altitude and is protected from the extremely cold climate due to a coat of very fine wool. These hairs are highly sought for weaving a shawl called shahtoosh. The large-scale poaching of the antelope has resulted in the species being placed on CITES Appendix I. We report on a method of DNA profiling on a confiscated shahtoosh using the cytochrome b (cyt b) gene to produce a test that will identify unambiguously the presence of P. hodgsonii. Two shahtoosh samples were provided by the Council of Agriculture, Taiwan, and ten shawl samples of sheep wool (Ovis aries), cashmere from the Kashmir goat (Capra hircus), and pashmina from the Himalayan goat (C. hircus) were collected from local stores for comparison. Two primer pairs were used to amplify a 271 bp fragment of cyt b gene which would distinguish these three species. The resulting amplification products were directly sequenced. When the DNA sequences were compared with the sequences registered in GenBank and EMBL databases, the sequences with the highest homology were the cyt b genes of P. hodgsonii, C. hircus, and O. aries. This study demonstrates that there is still sufficient DNA present in the finished wool of a shahtoosh shawl to allow DNA typing and the method established was highly plausible to identify the CITES protected species.

LanguageEnglish
Pages3359-3362
Number of pages4
JournalElectrophoresis
Volume27
DOIs
Publication statusPublished - 2006

Fingerprint

DNA Fingerprinting
Wool
Cytochromes b
Antelopes
Goats
Domestic Sheep
Genes
DNA
Cold Climate
DNA sequences
Nucleic Acid Databases
Agriculture
Taiwan
Hair
Amplification
Sheep
Animals
Databases
Population

Keywords

  • cytochrome b
  • species identification
  • shahtoosh
  • nested PCR

Cite this

Linacre, A. M. T., Lee, J. C., Tsai, L., Yang, C., Hsieh, H., Chiang, H., ... Huang, N. (2006). DNA profiling of shahtoosh. Electrophoresis, 27, 3359-3362. https://doi.org/10.1002/elps.200600062
Linacre, A.M.T. ; Lee, J.C. ; Tsai, L. ; Yang, C. ; Hsieh, H. ; Chiang, H. ; Lai, S. ; Huang, N. / DNA profiling of shahtoosh. In: Electrophoresis. 2006 ; Vol. 27. pp. 3359-3362.
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Linacre, AMT, Lee, JC, Tsai, L, Yang, C, Hsieh, H, Chiang, H, Lai, S & Huang, N 2006, 'DNA profiling of shahtoosh' Electrophoresis, vol. 27, pp. 3359-3362. https://doi.org/10.1002/elps.200600062

DNA profiling of shahtoosh. / Linacre, A.M.T.; Lee, J.C.; Tsai, L.; Yang, C.; Hsieh, H.; Chiang, H.; Lai, S.; Huang, N.

In: Electrophoresis, Vol. 27, 2006, p. 3359-3362.

Research output: Contribution to journalArticle

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ER -

Linacre AMT, Lee JC, Tsai L, Yang C, Hsieh H, Chiang H et al. DNA profiling of shahtoosh. Electrophoresis. 2006;27:3359-3362. https://doi.org/10.1002/elps.200600062