DNA duplexes stabilized by modified monomer residues: synthesis and stability

Research output: Contribution to journalArticle

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Abstract

The synthesis of a series of 2'-deoxyuridine analogues modified at the 5-position and a series of 2'-deoxypurines modified at the 8-position is described. Ultraviolet melting studies have been used to assess the stability of DNA duplexes 12- and 8-residues in length that contain these modifications. Of those modified residues which have been incorporated into oligonucleotides, 5-(prop-1-ynyl)-2'-deoxyuridine is found to impart the greatest increase in stability on the DNA duplexes studied.
LanguageEnglish
Pages1131-1138
Number of pages7
JournalJournal of the Chemical Society, Perkin Transactions 1
Volume1998
Issue number6
DOIs
Publication statusPublished - 21 Mar 1998

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Deoxyuridine
Monomers
DNA
Oligonucleotides
Melting

Keywords

  • substituted pyrimidine nucleosides
  • acid related-compounds
  • antiviral activity
  • oligonucleotides
  • oligodeoxynucleotides
  • 2'-deoxyuridine
  • derivatives
  • agents
  • cells

Cite this

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title = "DNA duplexes stabilized by modified monomer residues: synthesis and stability",
abstract = "The synthesis of a series of 2'-deoxyuridine analogues modified at the 5-position and a series of 2'-deoxypurines modified at the 8-position is described. Ultraviolet melting studies have been used to assess the stability of DNA duplexes 12- and 8-residues in length that contain these modifications. Of those modified residues which have been incorporated into oligonucleotides, 5-(prop-1-ynyl)-2'-deoxyuridine is found to impart the greatest increase in stability on the DNA duplexes studied.",
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DNA duplexes stabilized by modified monomer residues: synthesis and stability. / Graham, Duncan; Parkinson, John A.; Brown, Tom.

In: Journal of the Chemical Society, Perkin Transactions 1, Vol. 1998, No. 6, 21.03.1998, p. 1131-1138.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA duplexes stabilized by modified monomer residues: synthesis and stability

AU - Graham, Duncan

AU - Parkinson, John A.

AU - Brown, Tom

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AB - The synthesis of a series of 2'-deoxyuridine analogues modified at the 5-position and a series of 2'-deoxypurines modified at the 8-position is described. Ultraviolet melting studies have been used to assess the stability of DNA duplexes 12- and 8-residues in length that contain these modifications. Of those modified residues which have been incorporated into oligonucleotides, 5-(prop-1-ynyl)-2'-deoxyuridine is found to impart the greatest increase in stability on the DNA duplexes studied.

KW - substituted pyrimidine nucleosides

KW - acid related-compounds

KW - antiviral activity

KW - oligonucleotides

KW - oligodeoxynucleotides

KW - 2'-deoxyuridine

KW - derivatives

KW - agents

KW - cells

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