DNA-directed spatial assembly of photosynthetic light-harvesting proteins

Sarah L. Henry, Jamie M. Withers, Ishwar Singh, Jonathan M. Cooper, Alasdair W. Clark, Glenn A. Burley, Richard J. Cogdell

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
65 Downloads (Pure)

Abstract

This manuscript describes the surface immobilization of a light-harvesting complex to prescribed locations directed by the sequence-selective recognition of duplex DNA. An engineered light-harvesting complex (RC-LH1) derived from Rhodopseudomonas (Rps.) palustris containing the zinc finger (ZF) domain zif268 was prepared. The zif268 domain directed the binding of zfRC-LH1 to target double-stranded DNA sequences both in solution and when immobilized on lithographically defined micro-patterns. Excitation energy transfer from the carotenoids to the bacteriochlorophyll pigments within zfRC-LH1 confirmed that the functional and structural integrity of the complex is retained after surface immobilization.

Original languageEnglish
Pages (from-to)1359-1362
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume14
Issue number4
Early online date9 Dec 2015
DOIs
Publication statusPublished - 28 Jan 2016

Keywords

  • surface immobilization
  • light-harvesting complex
  • DNA
  • photosynthetic light-harvesting proteins

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