DNA-directed spatial assembly of photosynthetic light-harvesting proteins

Sarah L. Henry, Jamie M. Withers, Ishwar Singh, Jonathan M. Cooper, Alasdair W. Clark, Glenn A. Burley, Richard J. Cogdell

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This manuscript describes the surface immobilization of a light-harvesting complex to prescribed locations directed by the sequence-selective recognition of duplex DNA. An engineered light-harvesting complex (RC-LH1) derived from Rhodopseudomonas (Rps.) palustris containing the zinc finger (ZF) domain zif268 was prepared. The zif268 domain directed the binding of zfRC-LH1 to target double-stranded DNA sequences both in solution and when immobilized on lithographically defined micro-patterns. Excitation energy transfer from the carotenoids to the bacteriochlorophyll pigments within zfRC-LH1 confirmed that the functional and structural integrity of the complex is retained after surface immobilization.

LanguageEnglish
Pages1359-1362
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume14
Issue number4
Early online date9 Dec 2015
DOIs
Publication statusPublished - 28 Jan 2016

Fingerprint

immobilization
Immobilization
deoxyribonucleic acid
assembly
Rhodopseudomonas
Bacteriochlorophylls
proteins
Light
carotenoids
Excitation energy
DNA sequences
DNA
Energy Transfer
Zinc Fingers
Structural integrity
Carotenoids
pigments
Pigments
Energy transfer
integrity

Keywords

  • surface immobilization
  • light-harvesting complex
  • DNA
  • photosynthetic light-harvesting proteins

Cite this

Henry, Sarah L. ; Withers, Jamie M. ; Singh, Ishwar ; Cooper, Jonathan M. ; Clark, Alasdair W. ; Burley, Glenn A. ; Cogdell, Richard J. / DNA-directed spatial assembly of photosynthetic light-harvesting proteins. In: Organic and Biomolecular Chemistry. 2016 ; Vol. 14, No. 4. pp. 1359-1362.
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DNA-directed spatial assembly of photosynthetic light-harvesting proteins. / Henry, Sarah L.; Withers, Jamie M.; Singh, Ishwar; Cooper, Jonathan M.; Clark, Alasdair W.; Burley, Glenn A.; Cogdell, Richard J.

In: Organic and Biomolecular Chemistry, Vol. 14, No. 4, 28.01.2016, p. 1359-1362.

Research output: Contribution to journalArticle

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