DNA detection using enzymatic signal production and SERS

Jennifer A. Dougan; Douglas MacRae; Duncan Graham; Karen Faulds

doi:10.1039/c0cc05216g

DNA detection using enzymatic signal production and SERS

Jennifer A. Dougan, Douglas MacRae, Duncan Graham, Karen Faulds

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

A novel DNA assay is demonstrated which relies on the production of a surface enhanced resonance Raman scattering (SERS) signal following sequence specific hybridisation and enzyme hydrolysis. This method achieves high sensitivity and specificity that can be applied to the detection of unlabelled duplex DNA and is the first step towards a SERS based signal amplification approach.

Original language	English
Pages (from-to)	4649-4651
Number of pages	3
Journal	Chemical Communications (London)
Volume	47
Issue number	16
Early online date	15 Mar 2011
DOIs	https://doi.org/10.1039/c0cc05216g
Publication status	Published - 2011

Keywords

resonance ram-scattering
exonuclease
nanoparticles
fluorescence
lambda
TIC - Bionanotechnology

Access to Document

10.1039/c0cc05216g

Wolfson Merit Award: Responsive Nanoparticles for Multi-Modal Imaging
Graham, D. (Principal Investigator)
Royal Society
1/11/10 → 31/10/15
Project: Research
EXOSERRS - AMPLIFICATION FREE DIRECT GENOMIC SEQUENCE ANALYSIS BY OPTICAL SPECTROSCOPY
Faulds, K. (Principal Investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/10/07 → 31/10/11
Project: Research

Cite this

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title = "DNA detection using enzymatic signal production and SERS",

abstract = "A novel DNA assay is demonstrated which relies on the production of a surface enhanced resonance Raman scattering (SERS) signal following sequence specific hybridisation and enzyme hydrolysis. This method achieves high sensitivity and specificity that can be applied to the detection of unlabelled duplex DNA and is the first step towards a SERS based signal amplification approach.",

keywords = "resonance ram-scattering, exonuclease, nanoparticles, fluorescence, lambda, TIC - Bionanotechnology",

author = "Dougan, {Jennifer A.} and Douglas MacRae and Duncan Graham and Karen Faulds",

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doi = "10.1039/c0cc05216g",

language = "English",

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T1 - DNA detection using enzymatic signal production and SERS

AU - Dougan, Jennifer A.

AU - MacRae, Douglas

AU - Graham, Duncan

AU - Faulds, Karen

PY - 2011

Y1 - 2011

N2 - A novel DNA assay is demonstrated which relies on the production of a surface enhanced resonance Raman scattering (SERS) signal following sequence specific hybridisation and enzyme hydrolysis. This method achieves high sensitivity and specificity that can be applied to the detection of unlabelled duplex DNA and is the first step towards a SERS based signal amplification approach.

AB - A novel DNA assay is demonstrated which relies on the production of a surface enhanced resonance Raman scattering (SERS) signal following sequence specific hybridisation and enzyme hydrolysis. This method achieves high sensitivity and specificity that can be applied to the detection of unlabelled duplex DNA and is the first step towards a SERS based signal amplification approach.

KW - resonance ram-scattering

KW - exonuclease

KW - nanoparticles

KW - fluorescence

KW - lambda

KW - TIC - Bionanotechnology

U2 - 10.1039/c0cc05216g

DO - 10.1039/c0cc05216g

M3 - Article

SN - 0009-241X

VL - 47

SP - 4649

EP - 4651

JO - Chemical Communications (London)

JF - Chemical Communications (London)

IS - 16

ER -

DNA detection using enzymatic signal production and SERS

Abstract

Keywords

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Projects

Wolfson Merit Award: Responsive Nanoparticles for Multi-Modal Imaging

EXOSERRS - AMPLIFICATION FREE DIRECT GENOMIC SEQUENCE ANALYSIS BY OPTICAL SPECTROSCOPY

Cite this