DNA detection using enzymatic signal production and SERS

Jennifer A. Dougan, Douglas MacRae, Duncan Graham, Karen Faulds

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A novel DNA assay is demonstrated which relies on the production of a surface enhanced resonance Raman scattering (SERS) signal following sequence specific hybridisation and enzyme hydrolysis. This method achieves high sensitivity and specificity that can be applied to the detection of unlabelled duplex DNA and is the first step towards a SERS based signal amplification approach.

Original languageEnglish
Pages (from-to)4649-4651
Number of pages3
JournalChemical Communications (London)
Volume47
Issue number16
Early online date15 Mar 2011
DOIs
Publication statusPublished - 2011

Keywords

  • resonance ram-scattering
  • exonuclease
  • nanoparticles
  • fluorescence
  • lambda
  • TIC - Bionanotechnology

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