DNA detection by surface enhanced resonance Raman scattering (SERRS)

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

This Education article outlines the different ways in which surface enhanced resonance Raman scattering (SERRS) can be used for the detection of DNA. The use of various different SERRS detection strategies that have allowed both sensitive and selective detection to be obtained is covered. Detection of DNA by SERRS involves the use of a dye with the DNA, whether as an intercalator or by direct covalent attachment. This generates strong SERRS signals that indicate the presence of the specific DNA sequence. The SERRS detection of DNA in different molecular biological assays is also discussed.
LanguageEnglish
Pages1125-1131
Number of pages6
JournalAnalyst
Volume130
Issue number8
DOIs
Publication statusPublished - 2005

Fingerprint

Raman Spectrum Analysis
Raman scattering
DNA
scattering
Intercalating Agents
DNA sequences
Biological Assay
Assays
dye
Coloring Agents
Dyes
Education
detection
education
assay

Keywords

  • benzotriazole azo-dye
  • colloidal silver
  • deoxyribonucleic-acid
  • nucleic-acids
  • ft-sers
  • spectroscopy
  • gold
  • adsorption
  • pyridine
  • spectra

Cite this

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title = "DNA detection by surface enhanced resonance Raman scattering (SERRS)",
abstract = "This Education article outlines the different ways in which surface enhanced resonance Raman scattering (SERRS) can be used for the detection of DNA. The use of various different SERRS detection strategies that have allowed both sensitive and selective detection to be obtained is covered. Detection of DNA by SERRS involves the use of a dye with the DNA, whether as an intercalator or by direct covalent attachment. This generates strong SERRS signals that indicate the presence of the specific DNA sequence. The SERRS detection of DNA in different molecular biological assays is also discussed.",
keywords = "benzotriazole azo-dye, colloidal silver, deoxyribonucleic-acid, nucleic-acids, ft-sers, spectroscopy, gold, adsorption, pyridine, spectra",
author = "K. Faulds and W.E. Smith and D. Graham",
year = "2005",
doi = "10.1039/b500248f",
language = "English",
volume = "130",
pages = "1125--1131",
journal = "Analyst",
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}

DNA detection by surface enhanced resonance Raman scattering (SERRS). / Faulds, K.; Smith, W.E.; Graham, D.

In: Analyst, Vol. 130, No. 8, 2005, p. 1125-1131.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA detection by surface enhanced resonance Raman scattering (SERRS)

AU - Faulds, K.

AU - Smith, W.E.

AU - Graham, D.

PY - 2005

Y1 - 2005

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AB - This Education article outlines the different ways in which surface enhanced resonance Raman scattering (SERRS) can be used for the detection of DNA. The use of various different SERRS detection strategies that have allowed both sensitive and selective detection to be obtained is covered. Detection of DNA by SERRS involves the use of a dye with the DNA, whether as an intercalator or by direct covalent attachment. This generates strong SERRS signals that indicate the presence of the specific DNA sequence. The SERRS detection of DNA in different molecular biological assays is also discussed.

KW - benzotriazole azo-dye

KW - colloidal silver

KW - deoxyribonucleic-acid

KW - nucleic-acids

KW - ft-sers

KW - spectroscopy

KW - gold

KW - adsorption

KW - pyridine

KW - spectra

UR - http://www.rsc.org/ej/AN/2005/b500248f.pdf

UR - http://dx.doi.org/10.1039/b500248f

U2 - 10.1039/b500248f

DO - 10.1039/b500248f

M3 - Article

VL - 130

SP - 1125

EP - 1131

JO - Analyst

T2 - Analyst

JF - Analyst

SN - 0003-2654

IS - 8

ER -