DNA binding activity of the Escherichia coli nitric oxide sensor NorR suggests a conserved target sequence in diverse proteobacteria

Nicholas P Tucker, Benoît D'Autréaux, David J Studholme, Stephen Spiro, Ray Dixon

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The Escherichia coli nitric oxide sensor NorR was shown to bind to the promoter region of the norVW transcription unit, forming at least two distinct complexes detectable by gel retardation. Three binding sites for NorR and two integration host factor binding sites were identified in the norR-norV intergenic region. The derived consensus sequence for NorR binding sites was used to search for novel members of the E. coli NorR regulon and to show that NorR binding sites are partially conserved in other members of the proteobacteria.
LanguageEnglish
Pages6656-6660
Number of pages5
JournalJournal of Bacteriology
Volume186
Issue number19
DOIs
Publication statusPublished - 2004

Fingerprint

Proteobacteria
Conserved Sequence
Nitric Oxide
Binding Sites
Escherichia coli
DNA
Integration Host Factors
Regulon
Intergenic DNA
Consensus Sequence
Genetic Promoter Regions
Gels

Keywords

  • amino acid sequence
  • binding sites
  • conserved sequence
  • DNA
  • escherichia coli proteins
  • molecular sequence data
  • nitric oxide
  • proteobacteria
  • trans-activators

Cite this

Tucker, Nicholas P ; D'Autréaux, Benoît ; Studholme, David J ; Spiro, Stephen ; Dixon, Ray. / DNA binding activity of the Escherichia coli nitric oxide sensor NorR suggests a conserved target sequence in diverse proteobacteria. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 19. pp. 6656-6660.
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DNA binding activity of the Escherichia coli nitric oxide sensor NorR suggests a conserved target sequence in diverse proteobacteria. / Tucker, Nicholas P; D'Autréaux, Benoît; Studholme, David J; Spiro, Stephen; Dixon, Ray.

In: Journal of Bacteriology, Vol. 186, No. 19, 2004, p. 6656-6660.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA binding activity of the Escherichia coli nitric oxide sensor NorR suggests a conserved target sequence in diverse proteobacteria

AU - Tucker, Nicholas P

AU - D'Autréaux, Benoît

AU - Studholme, David J

AU - Spiro, Stephen

AU - Dixon, Ray

PY - 2004

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KW - amino acid sequence

KW - binding sites

KW - conserved sequence

KW - DNA

KW - escherichia coli proteins

KW - molecular sequence data

KW - nitric oxide

KW - proteobacteria

KW - trans-activators

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