Discovery of catalytic phages by biocatalytic self-assembly

Yoshiaki Maeda, Nadeem Javid, Krystyna Duncan, Louise Birchall, Kirsty F Gibson, Daniel Cannon, Yuka Kanetsuki, Charles W Knapp, Tell Tuttle, Rein V. Ulijn, Hiroshi Matsui

Research output: Contribution to journalArticle

28 Citations (Scopus)
136 Downloads (Pure)

Abstract

Discovery of new catalysts for demanding aqueous reactions is challenging. In here, we describe methodology for selection of catalytic phages by taking advantage of localized assembly of the product of the catalytic reaction that is screened for. A phage display library covering 109 unique dodecapeptide sequences is incubated with non-assembling precursors. Phages which are able to catalyze formation of the self-assembling reaction product (via amide condensation) acquire an aggregate of reaction product, enabling separation by centrifugation. The thus selected phages can be ampli-fied by infection of E.coli. These phages are shown to catalyze amide condensation and hydrolysis. Kinetic analysis shows a minor role for substrate binding. The approach enables discovery and mass-production of biocatalytic phages.

Original languageEnglish
Pages (from-to)15893–15896
Number of pages4
JournalJournal of the American Chemical Society
Volume136
Issue number45
Early online date24 Oct 2014
DOIs
Publication statusPublished - 2014

Keywords

  • catalysis
  • biocatalysis
  • esterase
  • amidase
  • phage display
  • self-assembly

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  • Cite this

    Maeda, Y., Javid, N., Duncan, K., Birchall, L., Gibson, K. F., Cannon, D., ... Matsui, H. (2014). Discovery of catalytic phages by biocatalytic self-assembly. Journal of the American Chemical Society, 136(45), 15893–15896. https://doi.org/10.1021/ja509393p