Direct surface-enhanced Raman scattering analysis of DNA duplexes

Luca Guerrini, Željka Krpetić, Danny van Lierop, Ramon A. Alvarez-Puebla, Duncan Graham

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The exploration of the genetic information carried by DNA has become a major scientific challenge. Routine DNA analysis, such as PCR, still suffers from important intrinsic limitations. Surface-enhanced Raman spectroscopy (SERS) has emerged as an outstanding opportunity for the development of DNA analysis, but its application to duplexes (dsDNA) has been largely hampered by reproducibility and/or sensitivity issues. A simple strategy is presented to perform ultrasensitive direct label-free analysis of unmodified dsDNA with the means of SERS by using positively charged silver colloids. Electrostatic adhesion of DNA promotes nanoparticle aggregation into stable clusters yielding intense and reproducible SERS spectra at nanogram level. As potential applications, we report the quantitative recognition of hybridization events as well as the first examples of SERS recognition of single base mismatches and base methylations (5-methylated cytosine and N6-methylated Adenine) in duplexes.

LanguageEnglish
Pages1144-1148
Number of pages5
JournalAngewandte Chemie International Edition
Volume54
Issue number4
DOIs
Publication statusPublished - 19 Jan 2015

Fingerprint

Raman spectroscopy
Raman scattering
DNA
Methylation
Cytosine
Adenine
Colloids
Labels
Electrostatics
Silver
Adhesion
Agglomeration
Nanoparticles

Keywords

  • DNA
  • hybridization
  • methylated bases
  • single-base mismatch
  • surface-enhanced raman spectroscopy

Cite this

Guerrini, Luca ; Krpetić, Željka ; van Lierop, Danny ; Alvarez-Puebla, Ramon A. ; Graham, Duncan. / Direct surface-enhanced Raman scattering analysis of DNA duplexes. In: Angewandte Chemie International Edition. 2015 ; Vol. 54, No. 4. pp. 1144-1148.
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Guerrini, L, Krpetić, Ž, van Lierop, D, Alvarez-Puebla, RA & Graham, D 2015, 'Direct surface-enhanced Raman scattering analysis of DNA duplexes' Angewandte Chemie International Edition, vol. 54, no. 4, pp. 1144-1148. https://doi.org/10.1002/anie.201408558

Direct surface-enhanced Raman scattering analysis of DNA duplexes. / Guerrini, Luca; Krpetić, Željka; van Lierop, Danny; Alvarez-Puebla, Ramon A.; Graham, Duncan.

In: Angewandte Chemie International Edition, Vol. 54, No. 4, 19.01.2015, p. 1144-1148.

Research output: Contribution to journalArticle

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