Direct observation of lipid hydroperoxides in phospholipid vesicles by electrospray mass spectrometry

C.M. Spickett, A.R. Pitt, Amanda J. Brown

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Positive ion electrospray ionization mass spectrometry was used to obtain a lipid profile of vesicles prepared from egg yolk lethicin and enriched with arachidonylstearoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine. The vesicles were oxidized by treatment with tert-butylhydroperoxide and iron (II) sulfate, and the formation of hydroperoxides of the polyunsaturated lipid arachidonylstearoyl phosphatidylcholine was observed. The native lipid signal at 832 a.m.u. decreased and new signals appeared at 864, 896, and 928 a.m.u., corresponding to the addition of one (+32), two (+64), and three (+96) molecules of dioxygen. The dihydroperoxide was found to be the most favourable peroxide product, but it appeared that a degradation of the hydroperoxides was occurring concomitant with their formation, and only their net formation was observed. The rate of depletion of the polyunsaturated lipid and the rate of accumulation of the hydroperoxides was found to increase with the Fe2+ concentration between 10 μM and 2 mM, and was also dependent on the tert-butylhydroperoxide concentration. This is the first report of analysis of lipid hydroperoxides by electrospray mass spectrometry, showing that technique offers a sensitive, direct, and informative approach to the study of oxidative damage to biological membranes.
LanguageEnglish
Pages613-620
Number of pages7
JournalFree Radical Biology and Medicine
Volume25
Issue number4-5
DOIs
Publication statusPublished - Sep 1998

Fingerprint

Lipid Peroxides
Mass spectrometry
tert-Butylhydroperoxide
Mass Spectrometry
Phospholipids
Observation
Hydrogen Peroxide
Phosphatidylcholines
Lipids
1,2-Dipalmitoylphosphatidylcholine
Egg Yolk
Electrospray Ionization Mass Spectrometry
Peroxides
Biological membranes
Electrospray ionization
Sulfates
Iron
Ions
Oxygen
Membranes

Keywords

  • lipid peroxidation
  • lipid hydroperoxides
  • electrospray ionization mass spectrometry
  • phosphatidylcholine
  • oxidative damage
  • free radical

Cite this

Spickett, C.M. ; Pitt, A.R. ; Brown, Amanda J. / Direct observation of lipid hydroperoxides in phospholipid vesicles by electrospray mass spectrometry. In: Free Radical Biology and Medicine. 1998 ; Vol. 25, No. 4-5. pp. 613-620.
@article{ba7fb046a1594542a3127f68d4a16f8f,
title = "Direct observation of lipid hydroperoxides in phospholipid vesicles by electrospray mass spectrometry",
abstract = "Positive ion electrospray ionization mass spectrometry was used to obtain a lipid profile of vesicles prepared from egg yolk lethicin and enriched with arachidonylstearoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine. The vesicles were oxidized by treatment with tert-butylhydroperoxide and iron (II) sulfate, and the formation of hydroperoxides of the polyunsaturated lipid arachidonylstearoyl phosphatidylcholine was observed. The native lipid signal at 832 a.m.u. decreased and new signals appeared at 864, 896, and 928 a.m.u., corresponding to the addition of one (+32), two (+64), and three (+96) molecules of dioxygen. The dihydroperoxide was found to be the most favourable peroxide product, but it appeared that a degradation of the hydroperoxides was occurring concomitant with their formation, and only their net formation was observed. The rate of depletion of the polyunsaturated lipid and the rate of accumulation of the hydroperoxides was found to increase with the Fe2+ concentration between 10 μM and 2 mM, and was also dependent on the tert-butylhydroperoxide concentration. This is the first report of analysis of lipid hydroperoxides by electrospray mass spectrometry, showing that technique offers a sensitive, direct, and informative approach to the study of oxidative damage to biological membranes.",
keywords = "lipid peroxidation, lipid hydroperoxides, electrospray ionization mass spectrometry, phosphatidylcholine, oxidative damage, free radical",
author = "C.M. Spickett and A.R. Pitt and Brown, {Amanda J.}",
year = "1998",
month = "9",
doi = "10.1016/S0891-5849(98)00074-4",
language = "English",
volume = "25",
pages = "613--620",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
number = "4-5",

}

Direct observation of lipid hydroperoxides in phospholipid vesicles by electrospray mass spectrometry. / Spickett, C.M.; Pitt, A.R.; Brown, Amanda J.

In: Free Radical Biology and Medicine, Vol. 25, No. 4-5, 09.1998, p. 613-620.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Direct observation of lipid hydroperoxides in phospholipid vesicles by electrospray mass spectrometry

AU - Spickett, C.M.

AU - Pitt, A.R.

AU - Brown, Amanda J.

PY - 1998/9

Y1 - 1998/9

N2 - Positive ion electrospray ionization mass spectrometry was used to obtain a lipid profile of vesicles prepared from egg yolk lethicin and enriched with arachidonylstearoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine. The vesicles were oxidized by treatment with tert-butylhydroperoxide and iron (II) sulfate, and the formation of hydroperoxides of the polyunsaturated lipid arachidonylstearoyl phosphatidylcholine was observed. The native lipid signal at 832 a.m.u. decreased and new signals appeared at 864, 896, and 928 a.m.u., corresponding to the addition of one (+32), two (+64), and three (+96) molecules of dioxygen. The dihydroperoxide was found to be the most favourable peroxide product, but it appeared that a degradation of the hydroperoxides was occurring concomitant with their formation, and only their net formation was observed. The rate of depletion of the polyunsaturated lipid and the rate of accumulation of the hydroperoxides was found to increase with the Fe2+ concentration between 10 μM and 2 mM, and was also dependent on the tert-butylhydroperoxide concentration. This is the first report of analysis of lipid hydroperoxides by electrospray mass spectrometry, showing that technique offers a sensitive, direct, and informative approach to the study of oxidative damage to biological membranes.

AB - Positive ion electrospray ionization mass spectrometry was used to obtain a lipid profile of vesicles prepared from egg yolk lethicin and enriched with arachidonylstearoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine. The vesicles were oxidized by treatment with tert-butylhydroperoxide and iron (II) sulfate, and the formation of hydroperoxides of the polyunsaturated lipid arachidonylstearoyl phosphatidylcholine was observed. The native lipid signal at 832 a.m.u. decreased and new signals appeared at 864, 896, and 928 a.m.u., corresponding to the addition of one (+32), two (+64), and three (+96) molecules of dioxygen. The dihydroperoxide was found to be the most favourable peroxide product, but it appeared that a degradation of the hydroperoxides was occurring concomitant with their formation, and only their net formation was observed. The rate of depletion of the polyunsaturated lipid and the rate of accumulation of the hydroperoxides was found to increase with the Fe2+ concentration between 10 μM and 2 mM, and was also dependent on the tert-butylhydroperoxide concentration. This is the first report of analysis of lipid hydroperoxides by electrospray mass spectrometry, showing that technique offers a sensitive, direct, and informative approach to the study of oxidative damage to biological membranes.

KW - lipid peroxidation

KW - lipid hydroperoxides

KW - electrospray ionization mass spectrometry

KW - phosphatidylcholine

KW - oxidative damage

KW - free radical

U2 - 10.1016/S0891-5849(98)00074-4

DO - 10.1016/S0891-5849(98)00074-4

M3 - Article

VL - 25

SP - 613

EP - 620

JO - Free Radical Biology and Medicine

T2 - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 4-5

ER -