Abstract
Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.
| Original language | English |
|---|---|
| Pages (from-to) | 8835-8841 |
| Number of pages | 7 |
| Journal | Journal of the American Chemical Society |
| Volume | 126 |
| Issue number | 28 |
| DOIs | |
| Publication status | Published - 21 Jul 2004 |
Keywords
- mediated fenton reactions
- hydrogen-peroxide
- oxidative stress
- oxygen radicals
- electrochemical oxidation
- strand breaks
- in-vivo
- iron
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