Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

L Dennany, R J Forster, B White, M Smyth, J F Rusling

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116 Citations (Scopus)

Abstract

Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

Original languageEnglish
Pages (from-to)8835-8841
Number of pages7
JournalJournal of the American Chemical Society
Volume126
Issue number28
DOIs
Publication statusPublished - 21 Jul 2004

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Keywords

  • mediated fenton reactions
  • hydrogen-peroxide
  • oxidative stress
  • oxygen radicals
  • electrochemical oxidation
  • strand breaks
  • in-vivo
  • iron

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