Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

L  Dennany; R J  Forster; B  White; M  Smyth; J F  Rusling

doi:10.1021/ja048615+

Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

L Dennany, R J Forster, B White, M Smyth, J F Rusling

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

133 Citations (Scopus)

Abstract

Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

Original language	English
Pages (from-to)	8835-8841
Number of pages	7
Journal	Journal of the American Chemical Society
Volume	126
Issue number	28
DOIs	https://doi.org/10.1021/ja048615+
Publication status	Published - 21 Jul 2004

Keywords

mediated fenton reactions
hydrogen-peroxide
oxidative stress
oxygen radicals
electrochemical oxidation
strand breaks
in-vivo
iron

Access to Document

10.1021/ja048615+

Cite this

@article{b5a1bfdd087e492286d664c0954906c3,

title = "Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)",

abstract = "Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.",

keywords = "mediated fenton reactions, hydrogen-peroxide, oxidative stress, oxygen radicals, electrochemical oxidation, strand breaks, in-vivo, iron",

author = "L Dennany and Forster, {R J} and B White and M Smyth and Rusling, {J F}",

year = "2004",

month = jul,

day = "21",

doi = "10.1021/ja048615+",

language = "English",

volume = "126",

pages = "8835--8841",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "28",

}

TY - JOUR

T1 - Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

AU - Dennany, L

AU - Forster, R J

AU - White, B

AU - Smyth, M

AU - Rusling, J F

PY - 2004/7/21

Y1 - 2004/7/21

N2 - Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

AB - Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

KW - mediated fenton reactions

KW - hydrogen-peroxide

KW - oxidative stress

KW - oxygen radicals

KW - electrochemical oxidation

KW - strand breaks

KW - in-vivo

KW - iron

U2 - 10.1021/ja048615+

DO - 10.1021/ja048615+

M3 - Article

SN - 0002-7863

VL - 126

SP - 8835

EP - 8841

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 28

ER -

Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

Abstract

Keywords

Access to Document

Fingerprint

Cite this