Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

L Dennany, R J Forster, B White, M Smyth, J F Rusling

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

Original languageEnglish
Pages (from-to)8835-8841
Number of pages7
JournalJournal of the American Chemical Society
Volume126
Issue number28
DOIs
Publication statusPublished - 21 Jul 2004

Fingerprint

Ultrathin films
DNA
Oligonucleotides
Guanine
Adenine
Pyridine
Polymers
Oxidation

Keywords

  • mediated fenton reactions
  • hydrogen-peroxide
  • oxidative stress
  • oxygen radicals
  • electrochemical oxidation
  • strand breaks
  • in-vivo
  • iron

Cite this

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title = "Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)",
abstract = "Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.",
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author = "L Dennany and Forster, {R J} and B White and M Smyth and Rusling, {J F}",
year = "2004",
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Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+). / Dennany, L ; Forster, R J ; White, B ; Smyth, M ; Rusling, J F .

In: Journal of the American Chemical Society, Vol. 126, No. 28, 21.07.2004, p. 8835-8841.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

AU - Dennany, L

AU - Forster, R J

AU - White, B

AU - Smyth, M

AU - Rusling, J F

PY - 2004/7/21

Y1 - 2004/7/21

N2 - Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

AB - Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

KW - mediated fenton reactions

KW - hydrogen-peroxide

KW - oxidative stress

KW - oxygen radicals

KW - electrochemical oxidation

KW - strand breaks

KW - in-vivo

KW - iron

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