Direct determination of ciprofloxacin by mass spectrometry after a two-step solid-phase extraction using a molecularly imprinted polymer

Ester Caro, Rosa M. Marcé, Peter A.G. Cormack, David C. Sherrington, Francesc Borrull

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A molecularly imprinted polymer (MIP) has been prepared for the first time with ciprofloxacin (CIPRO) as the template molecule, via a noncovalent synthetic procedure. Prior to its use as a sorbent in SPE, the MIP was evaluated chromatographically to confirm that it was indeed molecularly imprinted. The MIP was then used to extract CIPRO selectively from urine samples by means of a two-step SPE procedure in which a commercial Oasis cartridge and a molecularly imprinted SPE cartridge were combined in series. This approach allowed the matrix compounds present in the samples to be removed effectively. The urine extracts obtained after this two-step SPE procedure was applied were relatively clean compared to the original samples, and this made it possible to inject directly the extracts into a mass spectrometer and thus quantify CIPRO in urine samples at low levels and reduce the time of analysis.
LanguageEnglish
Pages1230-1236
Number of pages7
JournalJournal of Separation Science
Volume29
Issue number9
DOIs
Publication statusPublished - Jun 2006

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Ciprofloxacin
Mass spectrometry
Polymers
Mass spectrometers
Sorbents
Molecules

Keywords

  • fluorinated quinolones
  • human urine
  • mass spectrometry
  • molecularly imprinted polymer
  • solid-phase extraction

Cite this

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abstract = "A molecularly imprinted polymer (MIP) has been prepared for the first time with ciprofloxacin (CIPRO) as the template molecule, via a noncovalent synthetic procedure. Prior to its use as a sorbent in SPE, the MIP was evaluated chromatographically to confirm that it was indeed molecularly imprinted. The MIP was then used to extract CIPRO selectively from urine samples by means of a two-step SPE procedure in which a commercial Oasis cartridge and a molecularly imprinted SPE cartridge were combined in series. This approach allowed the matrix compounds present in the samples to be removed effectively. The urine extracts obtained after this two-step SPE procedure was applied were relatively clean compared to the original samples, and this made it possible to inject directly the extracts into a mass spectrometer and thus quantify CIPRO in urine samples at low levels and reduce the time of analysis.",
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Direct determination of ciprofloxacin by mass spectrometry after a two-step solid-phase extraction using a molecularly imprinted polymer. / Caro, Ester; Marcé, Rosa M.; Cormack, Peter A.G.; Sherrington, David C.; Borrull, Francesc.

In: Journal of Separation Science, Vol. 29, No. 9, 06.2006, p. 1230-1236.

Research output: Contribution to journalArticle

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T1 - Direct determination of ciprofloxacin by mass spectrometry after a two-step solid-phase extraction using a molecularly imprinted polymer

AU - Caro, Ester

AU - Marcé, Rosa M.

AU - Cormack, Peter A.G.

AU - Sherrington, David C.

AU - Borrull, Francesc

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KW - fluorinated quinolones

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KW - mass spectrometry

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