Differentiation and protease production in Micromonospora echinospora (ATCC 15837)

P. Hoskisson, G.P. Sharples, G. Hobbs

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Micromonospora echinospora differentiates in both submerged and surface cultures producing abundant dark spores after a period of vegetative mycelial growth. In submerged batch cultures, under either carbon or nitrogen limiting conditions, protease activity was found to coincide with sporulation indicating a relationship between proteolytic activity and differentiation in this organism. Further evidence for this link was provided from surface grown cultures wherein sporulation was inhibited by the serine protease inhibitors TLCK and TPCK. The association between proteolysis and differentiation apparent in this organism correlates with evidence of a similar phenomenon observed in the streptomycetes, suggesting that this may be a common response associated with differentiation in filamentous actinomycetes.
Original languageEnglish
Pages (from-to)191-195
Number of pages4
JournalAntonie van Leeuwenhoek
Volume89
Issue number1
DOIs
Publication statusPublished - 3 Dec 2005

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Tosyllysine Chloromethyl Ketone
Tosylphenylalanyl Chloromethyl Ketone
Micromonospora
Serine Proteinase Inhibitors
Batch Cell Culture Techniques
Actinobacteria
Spores
Proteolysis
Peptide Hydrolases
Nitrogen
Carbon
Growth

Keywords

  • protease production
  • micromonospora echinospora
  • sporulation
  • differentiation

Cite this

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Differentiation and protease production in Micromonospora echinospora (ATCC 15837). / Hoskisson, P.; Sharples, G.P.; Hobbs, G.

In: Antonie van Leeuwenhoek, Vol. 89, No. 1, 03.12.2005, p. 191-195.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Hoskisson, P.

AU - Sharples, G.P.

AU - Hobbs, G.

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KW - protease production

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