Differential responses in human subcutaneous and skeletal muscle vascular beds to critical limb ischaemia

P Coats, C Hillier

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objectives to investigate the effects of chronic ischaemia on the subcutaneous and the skeletal muscle resistance vasculature. To understand the redistribution of available blood in the ischaemic limb. Methods human subcutaneous and skeletal muscle resistance arteries were obtained from limbs amputated for critical limb ischaemia and studied under isobaric conditions using pressure myography. Morphological measurements of wall and lumen were analysed using light microscopy and image analysis. Vasoconstrictor responses to potassium and adrenoceptor agonists were used to measure functional status. Noradrenaline re-uptake mechanisms and α1-selectivity were investigated. Results both human skeletal muscle and subcutaneous resistance arteries undergo a severe atrophy of the arterial wall in ischaemic conditions. However, whereas subcutaneous resistance arteries become less able to vasoconstrict to adrenoceptor stimulation, the response of skeletal muscle resistance arteries becomes exaggerated and significantly augmented. This is true in response to both the endogenous vasoconstrictor noradraline and the α1-selective adrenoceptor agonist phenylephrine. Conclusions hypersensitivity to circulating catecholamines in the skeletal muscle vascular resistance bed may contribute to the progression of ischaemic disease by differentially diverting available blood to the subcutaneous tissue to the detriment of skeletal muscle perfusion.
LanguageEnglish
Pages387-395
Number of pages9
JournalEuropean Journal of Vascular and Endovascular Surgery
Volume19
Issue number4
DOIs
Publication statusPublished - Apr 2000

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Blood Vessels
Skeletal Muscle
Ischemia
Extremities
Arteries
Adrenergic Receptors
Vasoconstrictor Agents
Myography
Subcutaneous Tissue
Phenylephrine
Vascular Resistance
Atrophy
Catecholamines
Disease Progression
Microscopy
Norepinephrine
Potassium
Hypersensitivity
Perfusion
Light

Keywords

  • aged
  • amputation
  • arteries
  • biopsy
  • critical illness
  • diabetes mellitus, type 1
  • diabetes mellitus, type 2
  • diabetic angiopathies
  • female
  • humans
  • ischemia
  • leg
  • male
  • muscle, skeletal
  • myography
  • skin
  • vascular resistance

Cite this

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title = "Differential responses in human subcutaneous and skeletal muscle vascular beds to critical limb ischaemia",
abstract = "Objectives to investigate the effects of chronic ischaemia on the subcutaneous and the skeletal muscle resistance vasculature. To understand the redistribution of available blood in the ischaemic limb. Methods human subcutaneous and skeletal muscle resistance arteries were obtained from limbs amputated for critical limb ischaemia and studied under isobaric conditions using pressure myography. Morphological measurements of wall and lumen were analysed using light microscopy and image analysis. Vasoconstrictor responses to potassium and adrenoceptor agonists were used to measure functional status. Noradrenaline re-uptake mechanisms and α1-selectivity were investigated. Results both human skeletal muscle and subcutaneous resistance arteries undergo a severe atrophy of the arterial wall in ischaemic conditions. However, whereas subcutaneous resistance arteries become less able to vasoconstrict to adrenoceptor stimulation, the response of skeletal muscle resistance arteries becomes exaggerated and significantly augmented. This is true in response to both the endogenous vasoconstrictor noradraline and the α1-selective adrenoceptor agonist phenylephrine. Conclusions hypersensitivity to circulating catecholamines in the skeletal muscle vascular resistance bed may contribute to the progression of ischaemic disease by differentially diverting available blood to the subcutaneous tissue to the detriment of skeletal muscle perfusion.",
keywords = "aged, amputation, arteries, biopsy, critical illness, diabetes mellitus, type 1, diabetes mellitus, type 2, diabetic angiopathies, female, humans, ischemia, leg, male, muscle, skeletal, myography, skin, vascular resistance",
author = "P Coats and C Hillier",
year = "2000",
month = "4",
doi = "10.1053/ejvs.1999.1023",
language = "English",
volume = "19",
pages = "387--395",
journal = "European Journal of Vascular and Endovascular Surgery",
issn = "1078-5884",
number = "4",

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TY - JOUR

T1 - Differential responses in human subcutaneous and skeletal muscle vascular beds to critical limb ischaemia

AU - Coats, P

AU - Hillier, C

PY - 2000/4

Y1 - 2000/4

N2 - Objectives to investigate the effects of chronic ischaemia on the subcutaneous and the skeletal muscle resistance vasculature. To understand the redistribution of available blood in the ischaemic limb. Methods human subcutaneous and skeletal muscle resistance arteries were obtained from limbs amputated for critical limb ischaemia and studied under isobaric conditions using pressure myography. Morphological measurements of wall and lumen were analysed using light microscopy and image analysis. Vasoconstrictor responses to potassium and adrenoceptor agonists were used to measure functional status. Noradrenaline re-uptake mechanisms and α1-selectivity were investigated. Results both human skeletal muscle and subcutaneous resistance arteries undergo a severe atrophy of the arterial wall in ischaemic conditions. However, whereas subcutaneous resistance arteries become less able to vasoconstrict to adrenoceptor stimulation, the response of skeletal muscle resistance arteries becomes exaggerated and significantly augmented. This is true in response to both the endogenous vasoconstrictor noradraline and the α1-selective adrenoceptor agonist phenylephrine. Conclusions hypersensitivity to circulating catecholamines in the skeletal muscle vascular resistance bed may contribute to the progression of ischaemic disease by differentially diverting available blood to the subcutaneous tissue to the detriment of skeletal muscle perfusion.

AB - Objectives to investigate the effects of chronic ischaemia on the subcutaneous and the skeletal muscle resistance vasculature. To understand the redistribution of available blood in the ischaemic limb. Methods human subcutaneous and skeletal muscle resistance arteries were obtained from limbs amputated for critical limb ischaemia and studied under isobaric conditions using pressure myography. Morphological measurements of wall and lumen were analysed using light microscopy and image analysis. Vasoconstrictor responses to potassium and adrenoceptor agonists were used to measure functional status. Noradrenaline re-uptake mechanisms and α1-selectivity were investigated. Results both human skeletal muscle and subcutaneous resistance arteries undergo a severe atrophy of the arterial wall in ischaemic conditions. However, whereas subcutaneous resistance arteries become less able to vasoconstrict to adrenoceptor stimulation, the response of skeletal muscle resistance arteries becomes exaggerated and significantly augmented. This is true in response to both the endogenous vasoconstrictor noradraline and the α1-selective adrenoceptor agonist phenylephrine. Conclusions hypersensitivity to circulating catecholamines in the skeletal muscle vascular resistance bed may contribute to the progression of ischaemic disease by differentially diverting available blood to the subcutaneous tissue to the detriment of skeletal muscle perfusion.

KW - aged

KW - amputation

KW - arteries

KW - biopsy

KW - critical illness

KW - diabetes mellitus, type 1

KW - diabetes mellitus, type 2

KW - diabetic angiopathies

KW - female

KW - humans

KW - ischemia

KW - leg

KW - male

KW - muscle, skeletal

KW - myography

KW - skin

KW - vascular resistance

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DO - 10.1053/ejvs.1999.1023

M3 - Article

VL - 19

SP - 387

EP - 395

JO - European Journal of Vascular and Endovascular Surgery

T2 - European Journal of Vascular and Endovascular Surgery

JF - European Journal of Vascular and Endovascular Surgery

SN - 1078-5884

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