Differential regulation of protein subdomain activity with caged bivalent ligands

G. Mayer; J. Müller; T. Mack; D.F. Freitag; T. Höver; Bernd Pötzsch; A. Heckel

doi:10.1002/cbic.200800814

Differential regulation of protein subdomain activity with caged bivalent ligands

G. Mayer, J. Müller, T. Mack, D.F. Freitag, T. Höver, Bernd Pötzsch, A. Heckel

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Spatiotemporal modulation of individual protein subdomains with light as the trigger signal becomes possible by using bivalent aptamers and introducing photolabile 'caging groups' to switch individual aptamer modules ON or OFF differentially. To the best of our knowledge, this is the first study to show that it is possible to modulate individual domain activity in aptamers, and thus also domain activity in proteins, with light.

Original language	English
Pages (from-to)	654-657
Number of pages	3
Journal	Chembiochem
Volume	10
Issue number	4
DOIs	https://doi.org/10.1002/cbic.200800814
Publication status	Published - 2009

Keywords

aptamers
blood clotting
caged compounds
dna
molecular switches

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10.1002/cbic.200800814

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abstract = "Spatiotemporal modulation of individual protein subdomains with light as the trigger signal becomes possible by using bivalent aptamers and introducing photolabile 'caging groups' to switch individual aptamer modules ON or OFF differentially. To the best of our knowledge, this is the first study to show that it is possible to modulate individual domain activity in aptamers, and thus also domain activity in proteins, with light.",

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AU - Müller, J.

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AU - Höver, T.

AU - Pötzsch, Bernd

AU - Heckel, A.

PY - 2009

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AB - Spatiotemporal modulation of individual protein subdomains with light as the trigger signal becomes possible by using bivalent aptamers and introducing photolabile 'caging groups' to switch individual aptamer modules ON or OFF differentially. To the best of our knowledge, this is the first study to show that it is possible to modulate individual domain activity in aptamers, and thus also domain activity in proteins, with light.

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KW - blood clotting

KW - caged compounds

KW - dna

KW - molecular switches

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DO - 10.1002/cbic.200800814

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ER -

Differential regulation of protein subdomain activity with caged bivalent ligands

Abstract

Keywords

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