Differential regulation of protein subdomain activity with caged bivalent ligands

G. Mayer, J. Müller, T. Mack, D.F. Freitag, T. Höver, Bernd Pötzsch, A. Heckel

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Spatiotemporal modulation of individual protein subdomains with light as the trigger signal becomes possible by using bivalent aptamers and introducing photolabile 'caging groups' to switch individual aptamer modules ON or OFF differentially. To the best of our knowledge, this is the first study to show that it is possible to modulate individual domain activity in aptamers, and thus also domain activity in proteins, with light.
LanguageEnglish
Pages654-657
Number of pages3
JournalChembiochem
Volume10
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

Ligands
Light
Proteins
Switches
Modulation

Keywords

  • aptamers
  • blood clotting
  • caged compounds
  • dna
  • molecular switches

Cite this

Mayer, G., Müller, J., Mack, T., Freitag, D. F., Höver, T., Pötzsch, B., & Heckel, A. (2009). Differential regulation of protein subdomain activity with caged bivalent ligands. Chembiochem, 10(4), 654-657. https://doi.org/10.1002/cbic.200800814
Mayer, G. ; Müller, J. ; Mack, T. ; Freitag, D.F. ; Höver, T. ; Pötzsch, Bernd ; Heckel, A. / Differential regulation of protein subdomain activity with caged bivalent ligands. In: Chembiochem. 2009 ; Vol. 10, No. 4. pp. 654-657.
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Mayer, G, Müller, J, Mack, T, Freitag, DF, Höver, T, Pötzsch, B & Heckel, A 2009, 'Differential regulation of protein subdomain activity with caged bivalent ligands' Chembiochem, vol. 10, no. 4, pp. 654-657. https://doi.org/10.1002/cbic.200800814

Differential regulation of protein subdomain activity with caged bivalent ligands. / Mayer, G.; Müller, J.; Mack, T.; Freitag, D.F.; Höver, T.; Pötzsch, Bernd; Heckel, A.

In: Chembiochem, Vol. 10, No. 4, 2009, p. 654-657.

Research output: Contribution to journalArticle

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Mayer G, Müller J, Mack T, Freitag DF, Höver T, Pötzsch B et al. Differential regulation of protein subdomain activity with caged bivalent ligands. Chembiochem. 2009;10(4):654-657. https://doi.org/10.1002/cbic.200800814