TY - JOUR
T1 - Differential fate of erythromycin and beta-lactam resistance genes from swine lagoon waste under different aquatic conditions
AU - Knapp, Charles W
AU - Zhang, Wen
AU - Sturm, Belinda SM
AU - Graham, David W.
PY - 2010/1/11
Y1 - 2010/1/11
N2 - The attenuation and fate of erythromycin-resistance-methylase (erm) and extendedspectrum beta-lactamse (bla) genes were quantified over time in aquatic systems by adding 20-L swine waste to 11,300-L outdoor mesocosms that simulated receiving water conditions below intensive
agricultural operations. The units were prepared with two different light-exposure scenarios and included artificial substrates to assess gene movement into biofilms. Of eleven genes tested, only erm(B), erm(F), blaSHV and blaTEM were found in sufficient quantity for monitoring. The genes
disappeared rapidly from the water column and first-order water-column disappearance coefficients were calculated. However, detected gene levels became elevated in the biofilms within 2 days, but then disappeared overtime. Differences were observed between sunlight and dark treatments and among individual genes, suggesting that ecological and gene-specific factors play roles in the fate of these genes after release into the environment. Ultimately, this information will aid in generating better
predictive models for gene fate.
AB - The attenuation and fate of erythromycin-resistance-methylase (erm) and extendedspectrum beta-lactamse (bla) genes were quantified over time in aquatic systems by adding 20-L swine waste to 11,300-L outdoor mesocosms that simulated receiving water conditions below intensive
agricultural operations. The units were prepared with two different light-exposure scenarios and included artificial substrates to assess gene movement into biofilms. Of eleven genes tested, only erm(B), erm(F), blaSHV and blaTEM were found in sufficient quantity for monitoring. The genes
disappeared rapidly from the water column and first-order water-column disappearance coefficients were calculated. However, detected gene levels became elevated in the biofilms within 2 days, but then disappeared overtime. Differences were observed between sunlight and dark treatments and among individual genes, suggesting that ecological and gene-specific factors play roles in the fate of these genes after release into the environment. Ultimately, this information will aid in generating better
predictive models for gene fate.
KW - antibiotic resistance
KW - penicillin
KW - macrolide
KW - surface water
KW - aquatic pollution
UR - http://www.scopus.com/inward/record.url?scp=77949912728&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.envpol.2009.12.020
U2 - 10.1016/j.envpol.2009.12.020
DO - 10.1016/j.envpol.2009.12.020
M3 - Article
SN - 0269-7491
VL - 158
SP - 1506
EP - 1512
JO - Environmental Pollution
JF - Environmental Pollution
IS - 5
ER -