Differential fate of erythromycin and beta-lactam resistance genes from swine lagoon waste under different aquatic conditions

Charles W Knapp, Wen Zhang, Belinda SM Sturm, David W. Graham

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The attenuation and fate of erythromycin-resistance-methylase (erm) and extendedspectrum beta-lactamse (bla) genes were quantified over time in aquatic systems by adding 20-L swine waste to 11,300-L outdoor mesocosms that simulated receiving water conditions below intensive agricultural operations. The units were prepared with two different light-exposure scenarios and included artificial substrates to assess gene movement into biofilms. Of eleven genes tested, only erm(B), erm(F), blaSHV and blaTEM were found in sufficient quantity for monitoring. The genes disappeared rapidly from the water column and first-order water-column disappearance coefficients were calculated. However, detected gene levels became elevated in the biofilms within 2 days, but then disappeared overtime. Differences were observed between sunlight and dark treatments and among individual genes, suggesting that ecological and gene-specific factors play roles in the fate of these genes after release into the environment. Ultimately, this information will aid in generating better predictive models for gene fate.
LanguageEnglish
Pages1506-1512
Number of pages6
JournalEnvironmental Pollution
Volume158
Issue number5
DOIs
Publication statusPublished - 11 Jan 2010

Fingerprint

beta-Lactam Resistance
beta-Lactams
Erythromycin
Swine
Genes
Biofilms
Water
Lactams
Sunlight
Light
Monitoring

Keywords

  • antibiotic resistance
  • penicillin
  • macrolide
  • surface water
  • aquatic pollution

Cite this

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abstract = "The attenuation and fate of erythromycin-resistance-methylase (erm) and extendedspectrum beta-lactamse (bla) genes were quantified over time in aquatic systems by adding 20-L swine waste to 11,300-L outdoor mesocosms that simulated receiving water conditions below intensive agricultural operations. The units were prepared with two different light-exposure scenarios and included artificial substrates to assess gene movement into biofilms. Of eleven genes tested, only erm(B), erm(F), blaSHV and blaTEM were found in sufficient quantity for monitoring. The genes disappeared rapidly from the water column and first-order water-column disappearance coefficients were calculated. However, detected gene levels became elevated in the biofilms within 2 days, but then disappeared overtime. Differences were observed between sunlight and dark treatments and among individual genes, suggesting that ecological and gene-specific factors play roles in the fate of these genes after release into the environment. Ultimately, this information will aid in generating better predictive models for gene fate.",
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Differential fate of erythromycin and beta-lactam resistance genes from swine lagoon waste under different aquatic conditions. / Knapp, Charles W; Zhang, Wen; Sturm, Belinda SM; Graham, David W.

In: Environmental Pollution, Vol. 158, No. 5, 11.01.2010, p. 1506-1512.

Research output: Contribution to journalArticle

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AU - Zhang, Wen

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