Differential cAMP signaling at hippocampal output synapses

Christian Wozny, Nikolaus Maier, Pawel Fidzinski, Jörg Breustedt, Joachim Behr, Dietmar Schmitz

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

cAMP is a critical second messenger involved in synaptic transmission and synaptic plasticity. Here, we show that activation of the adenylyl cyclase by forskolin and application of the cAMP-analog Sp-5,6-DCl-cBIMPS both mimicked and occluded tetanus-induced long-term potentiation (LTP) in subicular bursting neurons, but not in subicular regular firing cells. Furthermore, LTP in bursting cells was inhibited by protein kinase A (PKA) inhibitors Rp-8-CPT-cAMP and H-89. Variations in the degree of EPSC blockade by the low-affinity competitive AMPA receptor-antagonist gamma-d-glutamyl-glycine (gamma-DGG), analysis of the coefficient of variance as well as changes in short-term potentiation suggest an increase of glutamate concentration in the synaptic cleft after expression of LTP. We conclude that presynaptic LTP in bursting cells requires activation of PKA by a calcium-dependent adenylyl cyclase while LTP in regular firing cells is independent of elevated cAMP levels. Our results provide evidence for a differential role of cAMP in LTP at hippocampal output synapses.

LanguageEnglish
Pages14358-14362
Number of pages5
JournalJournal of Neuroscience
Volume28
Issue number53
DOIs
Publication statusPublished - 31 Dec 2008

Fingerprint

Long-Term Potentiation
Synapses
Cyclic AMP-Dependent Protein Kinases
Adenylyl Cyclases
Neuronal Plasticity
AMPA Receptors
Tetanus
Second Messenger Systems
Colforsin
Protein Kinase Inhibitors
Synaptic Transmission
Glycine
Glutamic Acid
Analysis of Variance
Calcium
Neurons

Keywords

  • action potentials
  • analysis of variance
  • animals
  • Calcium
  • colforsin
  • cyclic AMP
  • electric stimulation
  • excitatory amino acid antagonists
  • excitatory postsynaptic potentials
  • GABA antagonists
  • hippocampus
  • in vitro techniques
  • isoquinolines
  • neurons
  • oligopeptides
  • patch-clamp techniques
  • protein kinase Inhibitors
  • pyridazines
  • quinoxalines
  • rats
  • signal transduction
  • sulfonamides
  • synapses
  • time factors

Cite this

Wozny, C., Maier, N., Fidzinski, P., Breustedt, J., Behr, J., & Schmitz, D. (2008). Differential cAMP signaling at hippocampal output synapses. Journal of Neuroscience, 28(53), 14358-14362. https://doi.org/10.1523/JNEUROSCI.4973-08.2008
Wozny, Christian ; Maier, Nikolaus ; Fidzinski, Pawel ; Breustedt, Jörg ; Behr, Joachim ; Schmitz, Dietmar. / Differential cAMP signaling at hippocampal output synapses. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 53. pp. 14358-14362.
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Wozny, C, Maier, N, Fidzinski, P, Breustedt, J, Behr, J & Schmitz, D 2008, 'Differential cAMP signaling at hippocampal output synapses' Journal of Neuroscience, vol. 28, no. 53, pp. 14358-14362. https://doi.org/10.1523/JNEUROSCI.4973-08.2008

Differential cAMP signaling at hippocampal output synapses. / Wozny, Christian; Maier, Nikolaus; Fidzinski, Pawel; Breustedt, Jörg; Behr, Joachim; Schmitz, Dietmar.

In: Journal of Neuroscience, Vol. 28, No. 53, 31.12.2008, p. 14358-14362.

Research output: Contribution to journalArticle

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T1 - Differential cAMP signaling at hippocampal output synapses

AU - Wozny, Christian

AU - Maier, Nikolaus

AU - Fidzinski, Pawel

AU - Breustedt, Jörg

AU - Behr, Joachim

AU - Schmitz, Dietmar

PY - 2008/12/31

Y1 - 2008/12/31

N2 - cAMP is a critical second messenger involved in synaptic transmission and synaptic plasticity. Here, we show that activation of the adenylyl cyclase by forskolin and application of the cAMP-analog Sp-5,6-DCl-cBIMPS both mimicked and occluded tetanus-induced long-term potentiation (LTP) in subicular bursting neurons, but not in subicular regular firing cells. Furthermore, LTP in bursting cells was inhibited by protein kinase A (PKA) inhibitors Rp-8-CPT-cAMP and H-89. Variations in the degree of EPSC blockade by the low-affinity competitive AMPA receptor-antagonist gamma-d-glutamyl-glycine (gamma-DGG), analysis of the coefficient of variance as well as changes in short-term potentiation suggest an increase of glutamate concentration in the synaptic cleft after expression of LTP. We conclude that presynaptic LTP in bursting cells requires activation of PKA by a calcium-dependent adenylyl cyclase while LTP in regular firing cells is independent of elevated cAMP levels. Our results provide evidence for a differential role of cAMP in LTP at hippocampal output synapses.

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KW - action potentials

KW - analysis of variance

KW - animals

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KW - electric stimulation

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KW - excitatory postsynaptic potentials

KW - GABA antagonists

KW - hippocampus

KW - in vitro techniques

KW - isoquinolines

KW - neurons

KW - oligopeptides

KW - patch-clamp techniques

KW - protein kinase Inhibitors

KW - pyridazines

KW - quinoxalines

KW - rats

KW - signal transduction

KW - sulfonamides

KW - synapses

KW - time factors

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SP - 14358

EP - 14362

JO - Journal of Neuroscience

T2 - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 53

ER -

Wozny C, Maier N, Fidzinski P, Breustedt J, Behr J, Schmitz D. Differential cAMP signaling at hippocampal output synapses. Journal of Neuroscience. 2008 Dec 31;28(53):14358-14362. https://doi.org/10.1523/JNEUROSCI.4973-08.2008