Dielectrophoretic manipulation of ribosomal RNA

G. Giraud, R. Pethig, H. Schulze, G. Henihan, J.G. Terry, A. Menachery, I. Ciani, D. Corrigan, C.J. Campbell, A.R. Mount, P. Ghazal, A.J. Walton, J. Crain, T.T. Bachmann

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The manipulation of ribosomal RNA(rRNA) extracted from E. coli cells by dielectrophoresis(DEP) has been demonstrated over the range of 3 kHz–50 MHz using interdigitated microelectrodes. Quantitative measurement using total internal reflection fluorescence microscopy of the time dependent collection indicated a positive DEP response characterized by a plateau between 3 kHz and 1 MHz followed by a decrease in response at higher frequencies. Negative DEP was observed above 9 MHz. The positive DEP response below 1 MHz is described by the Clausius–Mossotti model and corresponds to an induced dipole moment of 3300 D with a polarizability of 7.8×10−32 F m2. The negative DEP response above 9 MHz indicates that the rRNA molecules exhibit a net moment of −250 D, to give an effective permittivity value of 78.5 ε0, close to that of the aqueous suspending medium, and a relatively small surface conductance value of ∼0.1 nS. This suggests that our rRNA samples have a fairly open structure accessible to the surrounding water molecules, with counterions strongly bound to the charged phosphate groups in the rRNA backbone. These results are the first demonstration of DEP for fast capture and release of rRNA units, opening new opportunities for rRNA-based biosensing devices.
LanguageEnglish
Article number024116
Number of pages16
JournalBiomicrofluidics
Volume5
Issue number2
DOIs
Publication statusPublished - 28 Jun 2011

Fingerprint

ribonucleic acids
Ribosomal RNA
Electrophoresis
manipulators
Molecules
Microelectrodes
Fluorescence microscopy
Dipole moment
Fluorescence Microscopy
Escherichia coli
molecules
plateaus
phosphates
Phosphates
dipole moments
Permittivity
Demonstrations
permittivity
microscopy
moments

Keywords

  • biological electrophoresis
  • dielectrophoresis
  • RNA
  • fluorescence microscopy

Cite this

Giraud, G., Pethig, R., Schulze, H., Henihan, G., Terry, J. G., Menachery, A., ... Bachmann, T. T. (2011). Dielectrophoretic manipulation of ribosomal RNA. Biomicrofluidics, 5(2), [024116]. https://doi.org/10.1063/1.3604395
Giraud, G. ; Pethig, R. ; Schulze, H. ; Henihan, G. ; Terry, J.G. ; Menachery, A. ; Ciani, I. ; Corrigan, D. ; Campbell, C.J. ; Mount, A.R. ; Ghazal, P. ; Walton, A.J. ; Crain, J. ; Bachmann, T.T. / Dielectrophoretic manipulation of ribosomal RNA. In: Biomicrofluidics. 2011 ; Vol. 5, No. 2.
@article{aa379c8fac114e68be52ba360a487142,
title = "Dielectrophoretic manipulation of ribosomal RNA",
abstract = "The manipulation of ribosomal RNA(rRNA) extracted from E. coli cells by dielectrophoresis(DEP) has been demonstrated over the range of 3 kHz–50 MHz using interdigitated microelectrodes. Quantitative measurement using total internal reflection fluorescence microscopy of the time dependent collection indicated a positive DEP response characterized by a plateau between 3 kHz and 1 MHz followed by a decrease in response at higher frequencies. Negative DEP was observed above 9 MHz. The positive DEP response below 1 MHz is described by the Clausius–Mossotti model and corresponds to an induced dipole moment of 3300 D with a polarizability of 7.8×10−32 F m2. The negative DEP response above 9 MHz indicates that the rRNA molecules exhibit a net moment of −250 D, to give an effective permittivity value of 78.5 ε0, close to that of the aqueous suspending medium, and a relatively small surface conductance value of ∼0.1 nS. This suggests that our rRNA samples have a fairly open structure accessible to the surrounding water molecules, with counterions strongly bound to the charged phosphate groups in the rRNA backbone. These results are the first demonstration of DEP for fast capture and release of rRNA units, opening new opportunities for rRNA-based biosensing devices.",
keywords = "biological electrophoresis, dielectrophoresis, RNA, fluorescence microscopy",
author = "G. Giraud and R. Pethig and H. Schulze and G. Henihan and J.G. Terry and A. Menachery and I. Ciani and D. Corrigan and C.J. Campbell and A.R. Mount and P. Ghazal and A.J. Walton and J. Crain and T.T. Bachmann",
year = "2011",
month = "6",
day = "28",
doi = "10.1063/1.3604395",
language = "English",
volume = "5",
journal = "Biomicrofluidics",
issn = "1932-1058",
number = "2",

}

Giraud, G, Pethig, R, Schulze, H, Henihan, G, Terry, JG, Menachery, A, Ciani, I, Corrigan, D, Campbell, CJ, Mount, AR, Ghazal, P, Walton, AJ, Crain, J & Bachmann, TT 2011, 'Dielectrophoretic manipulation of ribosomal RNA' Biomicrofluidics, vol. 5, no. 2, 024116. https://doi.org/10.1063/1.3604395

Dielectrophoretic manipulation of ribosomal RNA. / Giraud, G.; Pethig, R.; Schulze, H.; Henihan, G.; Terry, J.G.; Menachery, A.; Ciani, I.; Corrigan, D.; Campbell, C.J.; Mount, A.R.; Ghazal, P.; Walton, A.J.; Crain, J.; Bachmann, T.T.

In: Biomicrofluidics, Vol. 5, No. 2, 024116, 28.06.2011.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dielectrophoretic manipulation of ribosomal RNA

AU - Giraud, G.

AU - Pethig, R.

AU - Schulze, H.

AU - Henihan, G.

AU - Terry, J.G.

AU - Menachery, A.

AU - Ciani, I.

AU - Corrigan, D.

AU - Campbell, C.J.

AU - Mount, A.R.

AU - Ghazal, P.

AU - Walton, A.J.

AU - Crain, J.

AU - Bachmann, T.T.

PY - 2011/6/28

Y1 - 2011/6/28

N2 - The manipulation of ribosomal RNA(rRNA) extracted from E. coli cells by dielectrophoresis(DEP) has been demonstrated over the range of 3 kHz–50 MHz using interdigitated microelectrodes. Quantitative measurement using total internal reflection fluorescence microscopy of the time dependent collection indicated a positive DEP response characterized by a plateau between 3 kHz and 1 MHz followed by a decrease in response at higher frequencies. Negative DEP was observed above 9 MHz. The positive DEP response below 1 MHz is described by the Clausius–Mossotti model and corresponds to an induced dipole moment of 3300 D with a polarizability of 7.8×10−32 F m2. The negative DEP response above 9 MHz indicates that the rRNA molecules exhibit a net moment of −250 D, to give an effective permittivity value of 78.5 ε0, close to that of the aqueous suspending medium, and a relatively small surface conductance value of ∼0.1 nS. This suggests that our rRNA samples have a fairly open structure accessible to the surrounding water molecules, with counterions strongly bound to the charged phosphate groups in the rRNA backbone. These results are the first demonstration of DEP for fast capture and release of rRNA units, opening new opportunities for rRNA-based biosensing devices.

AB - The manipulation of ribosomal RNA(rRNA) extracted from E. coli cells by dielectrophoresis(DEP) has been demonstrated over the range of 3 kHz–50 MHz using interdigitated microelectrodes. Quantitative measurement using total internal reflection fluorescence microscopy of the time dependent collection indicated a positive DEP response characterized by a plateau between 3 kHz and 1 MHz followed by a decrease in response at higher frequencies. Negative DEP was observed above 9 MHz. The positive DEP response below 1 MHz is described by the Clausius–Mossotti model and corresponds to an induced dipole moment of 3300 D with a polarizability of 7.8×10−32 F m2. The negative DEP response above 9 MHz indicates that the rRNA molecules exhibit a net moment of −250 D, to give an effective permittivity value of 78.5 ε0, close to that of the aqueous suspending medium, and a relatively small surface conductance value of ∼0.1 nS. This suggests that our rRNA samples have a fairly open structure accessible to the surrounding water molecules, with counterions strongly bound to the charged phosphate groups in the rRNA backbone. These results are the first demonstration of DEP for fast capture and release of rRNA units, opening new opportunities for rRNA-based biosensing devices.

KW - biological electrophoresis

KW - dielectrophoresis

KW - RNA

KW - fluorescence microscopy

UR - http://ShowEdit https://www.scopus.com/inward/record.uri?eid=2-s2.0-79959913728&partnerID=40&md5=7acb5c738fd270e1ff286a0f13b9028b

U2 - 10.1063/1.3604395

DO - 10.1063/1.3604395

M3 - Article

VL - 5

JO - Biomicrofluidics

T2 - Biomicrofluidics

JF - Biomicrofluidics

SN - 1932-1058

IS - 2

M1 - 024116

ER -

Giraud G, Pethig R, Schulze H, Henihan G, Terry JG, Menachery A et al. Dielectrophoretic manipulation of ribosomal RNA. Biomicrofluidics. 2011 Jun 28;5(2). 024116. https://doi.org/10.1063/1.3604395