Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)

Jan M. van der Wolf, Els H. Nijhuis, Malgorzata J. Kowalewska, Gerry S. Saddler, Neil Parkinson, John G. Elphinstone, Leighton Pritchard, Ian K. Toth, Ewa Lojkowska, Marta Potrykus, Malgorzata Waleron, Paul de Vos, Ilse Cleenwerck, Minna Pirhonen, Linda Garlant, Valérie Hélias, Joël F. Pothier, Valentin Pflüger, Brion Duffy, Leah Tsror & 1 others Shula Manulis

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously the Pectobacterium chrysanthemi complex (Erwinia chrysanthemi), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi, showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya. The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB,rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA-DNA hybridization studies, showed that although related to Dickeya dadantii, these isolates represent a novel species within the genus Dickeya, for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed.

LanguageEnglish
Pages768-774
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue number3
DOIs
Publication statusPublished - 1 Mar 2014

Fingerprint

Pectobacterium chrysanthemi
Dickeya
plant pathogenic bacteria
Erwinia chrysanthemi
Solanum tuberosum
potato
potatoes
Bacteria
bacterium
new species
Hyacinthus
DNA
Acetylesterase
Plant Diseases
Multilocus Sequence Typing
Intergenic DNA
Pulsed Field Gel Electrophoresis
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Israel
rRNA Genes

Keywords

  • potato plants
  • Pectobacterium chrysanthemi
  • Erwinia chrysanthemi
  • mass spectrometry

Cite this

van der Wolf, J. M., Nijhuis, E. H., Kowalewska, M. J., Saddler, G. S., Parkinson, N., Elphinstone, J. G., ... Manulis, S. (2014). Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum). International Journal of Systematic and Evolutionary Microbiology, 64(3), 768-774. https://doi.org/10.1099/ijs.0.052944-0
van der Wolf, Jan M. ; Nijhuis, Els H. ; Kowalewska, Malgorzata J. ; Saddler, Gerry S. ; Parkinson, Neil ; Elphinstone, John G. ; Pritchard, Leighton ; Toth, Ian K. ; Lojkowska, Ewa ; Potrykus, Marta ; Waleron, Malgorzata ; de Vos, Paul ; Cleenwerck, Ilse ; Pirhonen, Minna ; Garlant, Linda ; Hélias, Valérie ; Pothier, Joël F. ; Pflüger, Valentin ; Duffy, Brion ; Tsror, Leah ; Manulis, Shula. / Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum). In: International Journal of Systematic and Evolutionary Microbiology. 2014 ; Vol. 64, No. 3. pp. 768-774.
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van der Wolf, JM, Nijhuis, EH, Kowalewska, MJ, Saddler, GS, Parkinson, N, Elphinstone, JG, Pritchard, L, Toth, IK, Lojkowska, E, Potrykus, M, Waleron, M, de Vos, P, Cleenwerck, I, Pirhonen, M, Garlant, L, Hélias, V, Pothier, JF, Pflüger, V, Duffy, B, Tsror, L & Manulis, S 2014, 'Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)' International Journal of Systematic and Evolutionary Microbiology, vol. 64, no. 3, pp. 768-774. https://doi.org/10.1099/ijs.0.052944-0

Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum). / van der Wolf, Jan M.; Nijhuis, Els H.; Kowalewska, Malgorzata J.; Saddler, Gerry S.; Parkinson, Neil; Elphinstone, John G.; Pritchard, Leighton; Toth, Ian K.; Lojkowska, Ewa; Potrykus, Marta; Waleron, Malgorzata; de Vos, Paul; Cleenwerck, Ilse; Pirhonen, Minna; Garlant, Linda; Hélias, Valérie; Pothier, Joël F.; Pflüger, Valentin; Duffy, Brion; Tsror, Leah; Manulis, Shula.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. 3, 01.03.2014, p. 768-774.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)

AU - van der Wolf, Jan M.

AU - Nijhuis, Els H.

AU - Kowalewska, Malgorzata J.

AU - Saddler, Gerry S.

AU - Parkinson, Neil

AU - Elphinstone, John G.

AU - Pritchard, Leighton

AU - Toth, Ian K.

AU - Lojkowska, Ewa

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AU - Waleron, Malgorzata

AU - de Vos, Paul

AU - Cleenwerck, Ilse

AU - Pirhonen, Minna

AU - Garlant, Linda

AU - Hélias, Valérie

AU - Pothier, Joël F.

AU - Pflüger, Valentin

AU - Duffy, Brion

AU - Tsror, Leah

AU - Manulis, Shula

PY - 2014/3/1

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N2 - Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously the Pectobacterium chrysanthemi complex (Erwinia chrysanthemi), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi, showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya. The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB,rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA-DNA hybridization studies, showed that although related to Dickeya dadantii, these isolates represent a novel species within the genus Dickeya, for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed.

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KW - potato plants

KW - Pectobacterium chrysanthemi

KW - Erwinia chrysanthemi

KW - mass spectrometry

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