Developmental regulation of alternatively spliced acetyl-co a carboxylase alpha MRNAs encoding isozymes with or without an eight amino acid domain upstream of ser-1200 phosphorylation motif in the mammary gland

M.C. Barber, L. Pooley, M. Travers

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Expression of a variant acetyl-CoA carboxylase-alpha (ACC-alpha) mRNA encoding an isozyme either comprising (+24nt) or lacking (Delta24nt) an eight amino acid domain proximal to the Ser-1200 phosphorylation motif has been investigated in ovine and rat mammary tissue throughout pregnancy and lactation. The ratio of the Delta24nt mRNA: +24nt mRNA in ovine tissues varied from 0.1-0.25 (spleen, lung, muscle, heart, adipose tissue, brain) to 0.6-0.8 (pancreas, liver, kidney) to approximately 5.0 (lactating mammary gland). The sixfold increase in total ACC-alpha mRNA expression in mammary gland during lactation was due entirely to a tenfold increase in the level of the Delta24nt species as the level of expression of the +24nt species remained unaltered between pregnancy and lactation. This mode of expression of the +24nt and Delta24nt mRNAs was similar in rat mammary gland. Between day 20 of pregnancy and day 4 of lactation the ratio of the Delta24nt : +24nt mRNA increased from 2:1 to 10-20:1. Forced involution reduced the ratio of the two mRNAs to levels observed throughout pregnancy. Treatment of lactating rats with bromocryptine reduced the ratio of the Delta24nt : +24nt mRNA to relative levels observed after forced involution, suggesting that the exonic splicing responsible for the generation of the two mRNA isoforms is prolactin responsive.
Original languageEnglish
Pages (from-to)349-356
Number of pages8
JournalJournal of Molecular Endocrinology
Volume27
DOIs
Publication statusPublished - 2001

Keywords

  • rat mammary tissue
  • rat pregnancy
  • lactation

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