Development of three dimensional culture systems for embryonic stem cells

K. A. Meade, R. V. Ulijn, S. Downes, C. L. R. Merry

Research output: Contribution to journalMeeting abstract

Abstract

This meeting abstract discusses the development of three dimensional culture systems for embryonic stem cells. The capacity of pluripotent, self-renewing embryonic stem (ES) cells to differentiate into any cell type of the adult body holds great potential for their use in tissue engineering and cellular therapy.
However, current two dimensional (2D) culture techniques are hindering their large scale culture and efficient differentiation. Three dimensional (3D) scaffolds can mimic the in vivo micro-environment of ES cells, leading to sustained pluripotency and enhanced differentiation into mature cell phenotypes. We are focusing on the development and application of electrospun poly(lactic-co-glycolic acid) (PLGA) scaffolds and self assembling Fluorenylmethoxycarbonyl (Fmoc) peptide hydrogels to the culture of ES cells. Parameters for electrospinning 20% PLGA in Hexafluoroisopropanol (HFIP) have been established. The resulting non woven meshes were comprised of fibres rang-
ing between 0.2 and 1.5 mm in diameter. Mouse ES cells cultured on the surface of RGD functionalised dipeptide hydrogels formed colonies reminiscent of those observed in standard 2D culture conditions. Cells seeded within the gels remained viable after 24hours. Future work will focus on assessing the survival of ES cells within the Fmoc-dipeptide hydrogels over a longer time period, and the successful application of electrospun PLGA to ES cell culture. Long term aims include modification of both 3D culture environments with oligosaccharides or extracellular matrix proteins in order to better replicate the environment stem cells experience in vivo.
LanguageEnglish
Article numberP125
Pages838-838
Number of pages1
JournalTissue Engineering Part A
Volume14
Issue number5
DOIs
Publication statusPublished - May 2008
EventAnnual Tissue Engineering and Regenerative Medicine International Society European Chapter Meeting - Porto, Portugal
Duration: 22 Jun 200826 Jun 2008

Fingerprint

Embryonic Stem Cells
Stem cells
Cell culture
Hydrogels
Dipeptides
Scaffolds
Acids
Culture Techniques
Extracellular Matrix Proteins
Tissue Engineering
Oligosaccharides
Bioelectric potentials
Electrospinning
Stem Cells
Scaffolds (biology)
Cell Culture Techniques
Gels
Tissue engineering
Peptides
Phenotype

Keywords

  • embrionic stem cells
  • cellular therapy
  • self-renewing embryonic stem cells

Cite this

Meade, K. A. ; Ulijn, R. V. ; Downes, S. ; Merry, C. L. R. / Development of three dimensional culture systems for embryonic stem cells. In: Tissue Engineering Part A. 2008 ; Vol. 14, No. 5. pp. 838-838.
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Development of three dimensional culture systems for embryonic stem cells. / Meade, K. A.; Ulijn, R. V.; Downes, S.; Merry, C. L. R.

In: Tissue Engineering Part A, Vol. 14, No. 5, P125, 05.2008, p. 838-838.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Development of three dimensional culture systems for embryonic stem cells

AU - Meade, K. A.

AU - Ulijn, R. V.

AU - Downes, S.

AU - Merry, C. L. R.

PY - 2008/5

Y1 - 2008/5

N2 - This meeting abstract discusses the development of three dimensional culture systems for embryonic stem cells. The capacity of pluripotent, self-renewing embryonic stem (ES) cells to differentiate into any cell type of the adult body holds great potential for their use in tissue engineering and cellular therapy.However, current two dimensional (2D) culture techniques are hindering their large scale culture and efficient differentiation. Three dimensional (3D) scaffolds can mimic the in vivo micro-environment of ES cells, leading to sustained pluripotency and enhanced differentiation into mature cell phenotypes. We are focusing on the development and application of electrospun poly(lactic-co-glycolic acid) (PLGA) scaffolds and self assembling Fluorenylmethoxycarbonyl (Fmoc) peptide hydrogels to the culture of ES cells. Parameters for electrospinning 20% PLGA in Hexafluoroisopropanol (HFIP) have been established. The resulting non woven meshes were comprised of fibres rang-ing between 0.2 and 1.5 mm in diameter. Mouse ES cells cultured on the surface of RGD functionalised dipeptide hydrogels formed colonies reminiscent of those observed in standard 2D culture conditions. Cells seeded within the gels remained viable after 24hours. Future work will focus on assessing the survival of ES cells within the Fmoc-dipeptide hydrogels over a longer time period, and the successful application of electrospun PLGA to ES cell culture. Long term aims include modification of both 3D culture environments with oligosaccharides or extracellular matrix proteins in order to better replicate the environment stem cells experience in vivo.

AB - This meeting abstract discusses the development of three dimensional culture systems for embryonic stem cells. The capacity of pluripotent, self-renewing embryonic stem (ES) cells to differentiate into any cell type of the adult body holds great potential for their use in tissue engineering and cellular therapy.However, current two dimensional (2D) culture techniques are hindering their large scale culture and efficient differentiation. Three dimensional (3D) scaffolds can mimic the in vivo micro-environment of ES cells, leading to sustained pluripotency and enhanced differentiation into mature cell phenotypes. We are focusing on the development and application of electrospun poly(lactic-co-glycolic acid) (PLGA) scaffolds and self assembling Fluorenylmethoxycarbonyl (Fmoc) peptide hydrogels to the culture of ES cells. Parameters for electrospinning 20% PLGA in Hexafluoroisopropanol (HFIP) have been established. The resulting non woven meshes were comprised of fibres rang-ing between 0.2 and 1.5 mm in diameter. Mouse ES cells cultured on the surface of RGD functionalised dipeptide hydrogels formed colonies reminiscent of those observed in standard 2D culture conditions. Cells seeded within the gels remained viable after 24hours. Future work will focus on assessing the survival of ES cells within the Fmoc-dipeptide hydrogels over a longer time period, and the successful application of electrospun PLGA to ES cell culture. Long term aims include modification of both 3D culture environments with oligosaccharides or extracellular matrix proteins in order to better replicate the environment stem cells experience in vivo.

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KW - cellular therapy

KW - self-renewing embryonic stem cells

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JO - Tissue Engineering Part A

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ER -