Development of small-size tubular-flow continuous reactors for the analysis of operational stability of enzymes in low-water systems

D. Pirozzi, P.J. Halling

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A very small-scale continuous flow reactor has been designed for use with enzymes in organic media, particularly for operational stability studies. It is constructed from fairly inexpensive components, and typically uses 5 mg of catalyst and flow rates of 1 to 5 mL/h, so only small quantities of feedstock need to be handled. The design allows control of the thermodynamic water activity of the feed, and works with temperatures up to at least 80 degreesC. The reactor has been operated with both nonpolar (octane) and polar (4-methyl- pentan-2-one) solvents, and with the more viscous solvent-free reactant mixture. It has been applied to studies of the operational stability of lipases from Chromobacterium viscosum (lyophilized powder or polypropylene-adsorbed) and Rhizomucor miehei(Lipozyme) in different experimental conditions. Transesterification of geraniol and ethylcaproate has been adopted as a model transformation.
LanguageEnglish
Pages244-248
Number of pages4
JournalBiotechnology and Bioengineering
Volume72
Issue number2
DOIs
Publication statusPublished - 20 Jan 2001

Fingerprint

Enzyme Stability
Enzymes
Rhizomucor
Water
Polypropylenes
Transesterification
Lipases
Thermodynamics
Powders
Feedstocks
Thermodynamic properties
Flow rate
Catalysts
Temperature
octane
Lipozyme
Chromobacterium viscosum lipase
geraniol

Keywords

  • continuous enzymatic reactors
  • low-water enzymatic systems
  • lipases
  • operational stability
  • Organic-solvents
  • membrane reactor
  • esterification
  • lipase
  • transesterification
  • interesterification
  • efficiency
  • state

Cite this

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title = "Development of small-size tubular-flow continuous reactors for the analysis of operational stability of enzymes in low-water systems",
abstract = "A very small-scale continuous flow reactor has been designed for use with enzymes in organic media, particularly for operational stability studies. It is constructed from fairly inexpensive components, and typically uses 5 mg of catalyst and flow rates of 1 to 5 mL/h, so only small quantities of feedstock need to be handled. The design allows control of the thermodynamic water activity of the feed, and works with temperatures up to at least 80 degreesC. The reactor has been operated with both nonpolar (octane) and polar (4-methyl- pentan-2-one) solvents, and with the more viscous solvent-free reactant mixture. It has been applied to studies of the operational stability of lipases from Chromobacterium viscosum (lyophilized powder or polypropylene-adsorbed) and Rhizomucor miehei(Lipozyme) in different experimental conditions. Transesterification of geraniol and ethylcaproate has been adopted as a model transformation.",
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N2 - A very small-scale continuous flow reactor has been designed for use with enzymes in organic media, particularly for operational stability studies. It is constructed from fairly inexpensive components, and typically uses 5 mg of catalyst and flow rates of 1 to 5 mL/h, so only small quantities of feedstock need to be handled. The design allows control of the thermodynamic water activity of the feed, and works with temperatures up to at least 80 degreesC. The reactor has been operated with both nonpolar (octane) and polar (4-methyl- pentan-2-one) solvents, and with the more viscous solvent-free reactant mixture. It has been applied to studies of the operational stability of lipases from Chromobacterium viscosum (lyophilized powder or polypropylene-adsorbed) and Rhizomucor miehei(Lipozyme) in different experimental conditions. Transesterification of geraniol and ethylcaproate has been adopted as a model transformation.

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KW - continuous enzymatic reactors

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KW - Organic-solvents

KW - membrane reactor

KW - esterification

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KW - transesterification

KW - interesterification

KW - efficiency

KW - state

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