Development of biomaterials for cellular differentiation using a metabolomics approach

E. V. Alakpa, K. Burgess, V. Jayawarna, R. Ulijn, M. Dalby

Research output: Contribution to journalMeeting abstract

43 Citations (Scopus)

Abstract

Understanding how to mimic the physical characteristics of the extracellular matrix in vitro is an invaluable tool with regards to being able
to target stem cell differentiation along selective cell lineages. One such
physical characteristic is the innate elasticity of the cell substrate. Studies have shown that the rigidity of a substrate has considerable influence over cellular behaviourisms such as migration and differentiation.
Here we make use of liquid chromatography coupled to high mass
accuracy mass spectrometry (LC-MS) as an established method for
monitoring small changes in cell stasis. As such, this can be exploited
to envisage the cellular metabolome (the entire array of metabolites
that exist within a cell at any point in time). The interpretation of external cues by stem cells causes the activation or deactivation of several
proteins, consequently causing shifts in the metabolome. This effect
renders the metabolic profile of a cell to be highly indicative of its phenotype at that point in time. This is potentially of significance in stem
cell research as the cells are metabolically quiescent in their self-renewing state in their natural niches and the metabolome is thought to
become up regulated during differentiation. Investigating MSC metabolites during directed differentiation has enabled scrutiny of the cell
behaviour as phenotype is altered and has the potential to influence
the manner in which biomaterials are designed for cell culture in vitro.
LanguageEnglish
Pages238-238
Number of pages1
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume6
Issue numberspecial issue 1
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Metabolomics
Biocompatible Materials
Stem cells
Biomaterials
Metabolome
Liquid chromatography
Substrates
Metabolites
Cell culture
Rigidity
Mass spectrometry
Elasticity
Chemical activation
Stem Cells
Phenotype
Cell Lineage
Liquid Chromatography
Cues
Extracellular Matrix
Cell Differentiation

Keywords

  • biomaterials
  • cellular differentiation
  • metabolomics approach

Cite this

Alakpa, E. V. ; Burgess, K. ; Jayawarna, V. ; Ulijn, R. ; Dalby, M. / Development of biomaterials for cellular differentiation using a metabolomics approach. In: Journal of Tissue Engineering and Regenerative Medicine. 2012 ; Vol. 6, No. special issue 1. pp. 238-238.
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Development of biomaterials for cellular differentiation using a metabolomics approach. / Alakpa, E. V.; Burgess, K.; Jayawarna, V.; Ulijn, R.; Dalby, M.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 6, No. special issue 1, 09.2012, p. 238-238.

Research output: Contribution to journalMeeting abstract

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T1 - Development of biomaterials for cellular differentiation using a metabolomics approach

AU - Alakpa, E. V.

AU - Burgess, K.

AU - Jayawarna, V.

AU - Ulijn, R.

AU - Dalby, M.

PY - 2012/9

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AB - Understanding how to mimic the physical characteristics of the extracellular matrix in vitro is an invaluable tool with regards to being ableto target stem cell differentiation along selective cell lineages. One suchphysical characteristic is the innate elasticity of the cell substrate. Studies have shown that the rigidity of a substrate has considerable influence over cellular behaviourisms such as migration and differentiation.Here we make use of liquid chromatography coupled to high massaccuracy mass spectrometry (LC-MS) as an established method formonitoring small changes in cell stasis. As such, this can be exploitedto envisage the cellular metabolome (the entire array of metabolitesthat exist within a cell at any point in time). The interpretation of external cues by stem cells causes the activation or deactivation of severalproteins, consequently causing shifts in the metabolome. This effectrenders the metabolic profile of a cell to be highly indicative of its phenotype at that point in time. This is potentially of significance in stemcell research as the cells are metabolically quiescent in their self-renewing state in their natural niches and the metabolome is thought tobecome up regulated during differentiation. Investigating MSC metabolites during directed differentiation has enabled scrutiny of the cellbehaviour as phenotype is altered and has the potential to influencethe manner in which biomaterials are designed for cell culture in vitro.

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