Development of an electrochemical sensor for detecting circulating tumour DNA

Research output: Contribution to conferencePoster

Abstract

This project aims to develop an electrochemical point of care sensor that can accurately detect circulating tumour DNA using electrochemical methods. Carbon electrodes are simple and affordable, making them suitable for characterizing processes in electrochemistry. They are also chemically inert at negative potential ranges in all media giving them an advantage over metal electrodes. A PCR reaction for ctDNA was developed to amplify DNA from patients positive for the KRAS G12D mutation and PCR cycle numbers in relation to DNA yields was optimised. DNA hybridization measurements was used to analyse the electrochemical activity of unmodified electrodes and DNA modified electrodes at different concentrations and to determine if differential binding could be obtained from such samples. Both the single and multielectrode carbon electrodes were sensitive and selective. An electrochemical measurement protocol was established and the desired bands of amplicons were obtained after amplification of clinical samples. Cyclic voltammetry proved to be more sensitive for carbon electrodes. A clear dose response showing a decrease in peak current was observed as target DNA was bound with current time to result of approximately 3.5 hours.
Original languageEnglish
Number of pages1
Publication statusPublished - 5 Sep 2019
EventBioMedEng19 - London, United Kingdom
Duration: 5 Sep 20196 Sep 2019
https://www.biomedeng19.com/

Conference

ConferenceBioMedEng19
CountryUnited Kingdom
CityLondon
Period5/09/196/09/19
Internet address

Keywords

  • biosensors
  • electrochemical sensor
  • tumour DNA
  • circulating tumour DNA
  • electrochemistry
  • ctDNA

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