Development of an antimicrobial blended white LED system containing pulsed 405-nm LEDs for decontamination applications

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This study details the design, build and testing of a prototype antimicrobial blended white light unit containing pulsed red, yellow, green and 405nm LEDs. With a push for alternative methods of disinfection, optical methods have become a topic of interest. Ultra-violet (UV) light is widely known for its antimicrobial properties however; 405nm light has demonstrated significant antimicrobial properties against many common hospital acquired pathogens. In this study, a pulsed, blended, white-light prototype with a high content of 405 nm antimicrobial light, was designed, built and tested. Antimicrobial efficacy testing of the prototype was conducted using Staphylococcus aureus and Pseudomonas. aeruginosa, two bacteria which are common causes of hospital acquired infections. These were exposure to 3 different light outputs from the prototype and the surviving bacteria enumerated. Results showed that the mixed light output provided a much better CRI and light output under which to work. Also, the light output containing 405 nm light provided an antimicrobial effect, with decontamination of 103 CFUml-1 populations of both bacterial species. The other light content (red, yellow, green) had no beneficial or adverse effects on the antimicrobial properties of the 405nm light. The results suggest that with further development, it could be possible to produce an antimicrobial blended white light containing pulsed 405nm light that could supplement or even replace standard white lighting in certain environments.
Original languageEnglish
Title of host publicationDesign and Quality for Biomedical Technologies X
Place of PublicationBellingham, WA
Number of pages9
Publication statusPublished - 14 Mar 2017
EventDesign and Quality for Biomedical Technologies X - San Francisco, United States
Duration: 28 Jan 201729 Jan 2017

Publication series

NameProceedings of SPIE


ConferenceDesign and Quality for Biomedical Technologies X
Country/TerritoryUnited States
CitySan Francisco


  • 405nm light
  • pulsed LEDs
  • blending
  • disinfection
  • environmental contamination
  • antimicrobial
  • violet light
  • efficacy


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