Aims ES-62 is a well-studied anti-inflammatory molecule secreted by L4-adult stage Acanthocheilonema viteae. We maintain the lifecycle of A. viteae using Meriones lybicus as the definitive host. Here we investigated whether the full lifecycle could be maintained, and functional ES-62 produced, in a related jird species – Meriones shawi. Methods and Results Adult worms were produced in comparable numbers in the two species, but very few microfilariae (MF) were observed in the M. shawi bloodstream. M. shawi ES-62 produced ex vivo was functional and protective in a murine model of arthritis. Myeloid-derived cells from naïve and infected jirds of both species were compared with respect to ROS production and osteoclast generation and some differences between the two species in both the absence and presence of infection were observed. Conclusions The lifecycle of A. viteae cannot be successfully completed in M. shawi jirds but L3 stage worms develop to adulthood and produce functional ES-62. Preliminary investigation into jird immune responses suggests that infection can differentially modulate myeloid responses in the two species. However species-specific reagents are required to understand the complex interplay between A. viteae and its host and to explain the lack of circulating MF in infected M. shawi jirds.
- filarial nematode
- myeloid cell