Development of acanthocheilonema viteae in meriones shawi: absence of microfilariae and production of active ES-62

Felicity E. Lumb, James Doonan, Marlene Corbet, Miguel A Pineda, Margaret M. Harnett, William Harnett

Research output: Contribution to journalArticle

Abstract

Aims ES-62 is a well-studied anti-inflammatory molecule secreted by L4-adult stage Acanthocheilonema viteae. We maintain the lifecycle of A. viteae using Meriones lybicus as the definitive host. Here we investigated whether the full lifecycle could be maintained, and functional ES-62 produced, in a related jird species – Meriones shawi. Methods and Results Adult worms were produced in comparable numbers in the two species, but very few microfilariae (MF) were observed in the M. shawi bloodstream. M. shawi ES-62 produced ex vivo was functional and protective in a murine model of arthritis. Myeloid-derived cells from naïve and infected jirds of both species were compared with respect to ROS production and osteoclast generation and some differences between the two species in both the absence and presence of infection were observed. Conclusions The lifecycle of A. viteae cannot be successfully completed in M. shawi jirds but L3 stage worms develop to adulthood and produce functional ES-62. Preliminary investigation into jird immune responses suggests that infection can differentially modulate myeloid responses in the two species. However species-specific reagents are required to understand the complex interplay between A. viteae and its host and to explain the lack of circulating MF in infected M. shawi jirds.
Original languageEnglish
Article numbere12803
Number of pages6
JournalParasite Immunology
Early online date22 Oct 2020
DOIs
Publication statusE-pub ahead of print - 22 Oct 2020

Keywords

  • ES-62
  • filarial nematode
  • jird
  • microfilaria
  • myeloid cell
  • osteoclast

Cite this