Projects per year
Abstract
Protein S-acylation is a reversible post-translational modification that modulates the localization and function of many cellular proteins. S-acylation is mediated by a family of zinc finger DHHC (Asp-His-His-Cys) domain–containing (zDHHC) proteins encoded by 23 distinct ZDHHC genes in the human genome. These enzymes catalyze S-acylation in a two-step process involving "autoacylation" of the cysteine residue in the catalytic DHHC motif followed by transfer of the acyl chain to a substrate cysteine. S-acylation is essential for many fundamental physiological processes, and there is growing interest in zDHHC enzymes as novel drug targets for a range of disorders. However, there is currently a lack of chemical modulators of S-acylation either for use as tool compounds or for potential development for therapeutic purposes. Here, we developed and implemented a novel FRET-based high-throughput assay for the discovery of compounds that interfere with autoacylation of zDHHC2, an enzyme that is implicated in neuronal S-acylation pathways. Our screen of >350,000 compounds identified two related tetrazole-containing compounds (TTZ-1 and TTZ-2) that inhibited both zDHHC2 autoacylation and substrate S-acylation in cell-free systems. These compounds were also active in human embryonic kidney 293T cells, where they inhibited the S-acylation of two substrates (SNAP25 and PSD95 [postsynaptic density protein 95]) mediated by different zDHHC enzymes, with some apparent isoform selectivity. Furthermore, we confirmed activity of the hit compounds through resynthesis, which provided sufficient quantities of material for further investigations. The assays developed provide novel strategies to screen for zDHHC inhibitors, and the identified compounds add to the chemical toolbox for interrogating cellular activities of zDHHC enzymes in S-acylation.
Original language | English |
---|---|
Article number | 102469 |
Number of pages | 22 |
Journal | Journal of Biological Chemistry |
Volume | 298 |
Issue number | 10 |
Early online date | 7 Oct 2022 |
DOIs | |
Publication status | Published - 31 Oct 2022 |
Keywords
- S-acylation
- zDHHC
- palmitoylation
- Inhibitor
- HTS
Fingerprint
Dive into the research topics of 'Development of a novel high-throughput screen for the identification of new inhibitors of protein S-acylation'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Identification of inhibitors of S-acylation
Salaun, C., Chamberlain, L., Tomkinson, N., Bushell, T., Galindo, A., Munro, K. R. & McLellan, J.
1/07/15 → 1/03/18
Project: Knowledge Exchange
Activities
- 1 Hosting an external, non-academic visitor
-
Hiroya Takizawa
Christine Salaun (Host), Luke Chamberlain (Host) & Nick Tomkinson (Host)
22 Feb 2016 → 26 Feb 2016Activity: Hosting a visitor types › Hosting an external, non-academic visitor