Development of a low-cost method of analysis for the qualitative and quantitative analysis of butyltins in environmental samples.

S. Bangkedphol, H.E. Keenan, C.M. Davidson, A. Sakultantimetha, A. Songsasen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Most analytical methods for butyltins are based on high resolution techniques with complicated sample preparation. For this study, a simple application of an analytical method was developed using High Performance Liquid Chromatography (HPLC) with UV detection. The developed method was studied to determine tributyltin (TBT), dibutyltin (DBT) and monobutyltin (MBT) in sediment and water samples. The separation was performed in isocratic mode on an ultra cyanopropyl column with a mobile phase of hexane containing 5% THF and 0.03% acetic acid. This method was confirmed using standard GC/MS techniques and verified by statistical paired t-test method. Under the experimental conditions used, the limit of detection (LOD) of TBT and DBT were 0.70 and 0.50 microg/mL, respectively. The optimised extraction method for butyltins in water and sediment samples involved using hexane containing 0.05-0.5% tropolone and 0.2% sodium chloride in water at pH 1.7. The quantitative extraction of butyltin compounds in a certified reference material (BCR-646) and naturally contaminated samples was achieved with recoveries ranging from 95 to 108% and at %RSD 0.02-1.00%. This HPLC method and optimum extraction conditions were used to determine the contamination level of butyltins in environmental samples collected from the Forth and Clyde canal, Scotland, UK. The values obtained severely exceeded the Environmental Quality Standard (EQS) values. Although high resolution methods are utilised extensively for this type of research, the developed method is cheaper in both terms of equipment and running costs, faster in analysis time and has comparable detection limits to the alternative methods. This is advantageous not just as a confirmatory technique but also to enable further research in this field.
LanguageEnglish
Pages1744-1551
Number of pages193
JournalJournal of Environmental Science and Health Part A
Volume43
Issue number14
DOIs
Publication statusPublished - Dec 2008

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High performance liquid chromatography
Hexane
Sediments
Chemical analysis
Costs
Water
Canals
Sodium chloride
Acetic acid
Contamination
Recovery

Keywords

  • tributyltin
  • dibutyltin
  • monobutyltin
  • butyltins
  • normal phase
  • HPLC
  • forth and clyde canal
  • EQS values

Cite this

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abstract = "Most analytical methods for butyltins are based on high resolution techniques with complicated sample preparation. For this study, a simple application of an analytical method was developed using High Performance Liquid Chromatography (HPLC) with UV detection. The developed method was studied to determine tributyltin (TBT), dibutyltin (DBT) and monobutyltin (MBT) in sediment and water samples. The separation was performed in isocratic mode on an ultra cyanopropyl column with a mobile phase of hexane containing 5{\%} THF and 0.03{\%} acetic acid. This method was confirmed using standard GC/MS techniques and verified by statistical paired t-test method. Under the experimental conditions used, the limit of detection (LOD) of TBT and DBT were 0.70 and 0.50 microg/mL, respectively. The optimised extraction method for butyltins in water and sediment samples involved using hexane containing 0.05-0.5{\%} tropolone and 0.2{\%} sodium chloride in water at pH 1.7. The quantitative extraction of butyltin compounds in a certified reference material (BCR-646) and naturally contaminated samples was achieved with recoveries ranging from 95 to 108{\%} and at {\%}RSD 0.02-1.00{\%}. This HPLC method and optimum extraction conditions were used to determine the contamination level of butyltins in environmental samples collected from the Forth and Clyde canal, Scotland, UK. The values obtained severely exceeded the Environmental Quality Standard (EQS) values. Although high resolution methods are utilised extensively for this type of research, the developed method is cheaper in both terms of equipment and running costs, faster in analysis time and has comparable detection limits to the alternative methods. This is advantageous not just as a confirmatory technique but also to enable further research in this field.",
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Development of a low-cost method of analysis for the qualitative and quantitative analysis of butyltins in environmental samples. / Bangkedphol, S.; Keenan, H.E.; Davidson, C.M.; Sakultantimetha, A.; Songsasen, A.

In: Journal of Environmental Science and Health Part A, Vol. 43, No. 14, 12.2008, p. 1744-1551.

Research output: Contribution to journalArticle

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T1 - Development of a low-cost method of analysis for the qualitative and quantitative analysis of butyltins in environmental samples.

AU - Bangkedphol, S.

AU - Keenan, H.E.

AU - Davidson, C.M.

AU - Sakultantimetha, A.

AU - Songsasen, A.

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KW - monobutyltin

KW - butyltins

KW - normal phase

KW - HPLC

KW - forth and clyde canal

KW - EQS values

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