Development of a hormone neutralizing vaccine, using GnRH-glycys-PPD, for use in the treatment of oestrogen-dependent disorders

V A Ferro, J E O'Grady, J Notman, W H Stimson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of this study was to develop an effective and nontoxic vaccine, suitable for use in humans, which was capable of effectively controlling oestrogen levels. Female Sprague-Dawley rats were immunized with a conjugated analogue of gonadotrophin releasing hormone, GnRH-glycys-PPD. This resulted in high levels of neutralizing antibody which disrupted GnRH function and consequently caused a reduction in serum oestrogen. The effect of oestrogen deprivation correlated well with ovarian failure and gonadal atrophy. An examination was made of various adjuvants in conjunction with the analogue to determine the suitability of the combinations in the formulation of an effective human vaccine. This investigation included a novel adjuvant, non-ionic surfactant vesicles (NISV); the results showed that NISV are completely nontoxic and in terms of potentiating and sustaining an immune response, compare favourably with Freund's adjuvant and alum. In addition the long term effects of immunization were investigated and the data showed that immunoneutralization of GnRH effectively suppresses fertility on a long-term basis.

LanguageEnglish
Pages147-157
Number of pages11
JournalTherapeutic immunology
Volume2
Issue number3
Publication statusPublished - 30 Jun 1995

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Tuberculin
Gonadotropin-Releasing Hormone
Estrogens
Vaccines
Hormones
Surface-Active Agents
Freund's Adjuvant
Therapeutics
Neutralizing Antibodies
Atrophy
Fertility
Sprague Dawley Rats
Immunization
Serum

Keywords

  • vaccine
  • hormone neutralizing vaccine
  • oestrogen-dependent disorders
  • oestrogen levels

Cite this

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Development of a hormone neutralizing vaccine, using GnRH-glycys-PPD, for use in the treatment of oestrogen-dependent disorders. / Ferro, V A; O'Grady, J E; Notman, J; Stimson, W H.

In: Therapeutic immunology, Vol. 2, No. 3, 30.06.1995, p. 147-157.

Research output: Contribution to journalArticle

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