TY - JOUR
T1 - Development of a high-accuracy, low-cost, and portable fluorometer with smartphone application for the detection of urinary albumin towards the early screening of chronic kidney and renal diseases
AU - Pinrod, Visarute
AU - Chawjiraphan, Wireeya
AU - Segkhoonthod, Khoonsake
AU - Hanchaisri, Kriangkai
AU - Tantiwathanapong, Phornpol
AU - Pinpradup, Preedee
AU - Putnin, Thitirat
AU - Pimalai, Dechnarong
AU - Treerattrakoon, Kiatnida
AU - Cha’on, Ubon
AU - Anutrakulchai, Sirirat
AU - Japrung, Deanpen
PY - 2023/9/8
Y1 - 2023/9/8
N2 - This study presents the development of a portable fluorometer with a smartphone application designed to facilitate the early screening of chronic kidney and renal diseases by enabling the sensitive detection of urinary albumin. Utilizing a fluorescence-based aptasensor, the device achieved a linear calibration curve (0.001–1.5 mg/mL) with a linearity of up to 0.98022 and a detection limit of 0.203 µg/mL for human serum albumin (HSA). The analysis of 130 urine samples demonstrated comparable performance between this study’s fluorometer, a commercial fluorometer, and the standard automated method. These findings validate the feasibility of the portable fluorometer and aptasensor combination as a reliable instrument for the sensitive and specific measurement of HSA in urine samples. Moreover, the fluorometer’s portability offers potential applications in portable point-of-care testing, enhancing its utility in clinical settings for early disease screening.
AB - This study presents the development of a portable fluorometer with a smartphone application designed to facilitate the early screening of chronic kidney and renal diseases by enabling the sensitive detection of urinary albumin. Utilizing a fluorescence-based aptasensor, the device achieved a linear calibration curve (0.001–1.5 mg/mL) with a linearity of up to 0.98022 and a detection limit of 0.203 µg/mL for human serum albumin (HSA). The analysis of 130 urine samples demonstrated comparable performance between this study’s fluorometer, a commercial fluorometer, and the standard automated method. These findings validate the feasibility of the portable fluorometer and aptasensor combination as a reliable instrument for the sensitive and specific measurement of HSA in urine samples. Moreover, the fluorometer’s portability offers potential applications in portable point-of-care testing, enhancing its utility in clinical settings for early disease screening.
KW - albuminuria
KW - aptasensor
KW - fluorescence quenching
KW - portable fluorometer
KW - chronic kidney disease
KW - renal disease
U2 - 10.3390/bios13090876
DO - 10.3390/bios13090876
M3 - Article
SN - 2079-6374
VL - 13
JO - Biosensors
JF - Biosensors
IS - 9
M1 - 876
ER -
