Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

Kay McMillan; Marie Boyd; Michele Zagnoni

Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

Kay McMillan, Marie Boyd, Michele Zagnoni

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

Abstract

In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma
cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent
surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.

Original language	English
Title of host publication	MicroTAS 2014
Place of Publication	San Diego, CA.
Pages	923-925
Number of pages	3
ISBN (Electronic)	9780979806476
Publication status	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014) - Texas, San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
Country/Territory	United States
City	San Antonio
Period	26/10/14 → 30/10/14

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cancer
spheroids
radiotherapy
droplet microfluidics
3D cell culture

Cite this

@inproceedings{dfedda13457e4d8ebf0ddf6699e18025,

title = "Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids",

abstract = "In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.",

keywords = "cancer, spheroids, radiotherapy, droplet microfluidics, 3D cell culture",

author = "Kay McMillan and Marie Boyd and Michele Zagnoni",

year = "2014",

language = "English",

pages = "923--925",

booktitle = "MicroTAS 2014",

note = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014) ; Conference date: 26-10-2014 Through 30-10-2014",

}

TY - GEN

T1 - Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

AU - McMillan, Kay

AU - Boyd, Marie

AU - Zagnoni, Michele

PY - 2014

Y1 - 2014

N2 - In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.

AB - In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.

KW - cancer

KW - spheroids

KW - radiotherapy

KW - droplet microfluidics

KW - 3D cell culture

UR - http://www.microtasconferences.org/microtas2014/

UR - http://www.rsc.org/images/loc/2014/PDFs/Papers/303_0574.pdf

M3 - Conference contribution book

SP - 923

EP - 925

BT - MicroTAS 2014

CY - San Diego, CA.

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)

Y2 - 26 October 2014 through 30 October 2014

ER -

Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

Abstract

Conference

UN SDGs

Keywords

Other files and links

Fingerprint

Epsrc Doctoral Training Grant | McMillan, Kay

advisor for microfluidic platform for development of spheroids

advisor on microfluidic spheroid platform development

Cite this

Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

Abstract

Conference

UN SDGs

Keywords

Other files and links

Fingerprint

Projects

Epsrc Doctoral Training Grant | McMillan, Kay

Activities

advisor for microfluidic platform for development of spheroids

advisor on microfluidic spheroid platform development

Cite this