Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

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Abstract

In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma
cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent
surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.
Original languageEnglish
Title of host publicationMicroTAS 2014
Place of PublicationSan Diego, CA.
Pages923-925
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014) - Texas, San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
CountryUnited States
CitySan Antonio
Period26/10/1430/10/14

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Keywords

  • cancer
  • spheroids
  • radiotherapy
  • droplet microfluidics
  • 3D cell culture

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