Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma
cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent
surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.
LanguageEnglish
Title of host publicationMicroTAS 2014
Place of PublicationSan Diego, CA.
Pages923-925
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014) - Texas, San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
CountryUnited States
CitySan Antonio
Period26/10/1430/10/14

Fingerprint

Cellular Spheroids
Microfluidics
Radiotherapy
Assays
Surface-Active Agents
Microscopy
Oils
Cell Count
Encapsulation
Dosimetry
Microscopic examination
Therapeutics
Growth

Keywords

  • cancer
  • spheroids
  • radiotherapy
  • droplet microfluidics
  • 3D cell culture

Cite this

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title = "Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids",
abstract = "In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Gliomacells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.",
keywords = "cancer, spheroids, radiotherapy, droplet microfluidics, 3D cell culture",
author = "Kay McMillan and Marie Boyd and Michele Zagnoni",
year = "2014",
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pages = "923--925",
booktitle = "MicroTAS 2014",

}

McMillan, K, Boyd, M & Zagnoni, M 2014, Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids. in MicroTAS 2014. San Diego, CA., pp. 923-925, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, United States, 26/10/14.

Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids. / McMillan, Kay; Boyd, Marie; Zagnoni, Michele.

MicroTAS 2014. San Diego, CA., 2014. p. 923-925.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

TY - GEN

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AU - Boyd, Marie

AU - Zagnoni, Michele

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AB - In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Gliomacells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.

KW - cancer

KW - spheroids

KW - radiotherapy

KW - droplet microfluidics

KW - 3D cell culture

UR - http://www.microtasconferences.org/microtas2014/

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