Abstract
Isometamidium, a mixture of related substances of which 8-(3-m-amidinophenyl-2-triazeno)-3-amino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B4180A) is the principal active component, is the only chemical agent available for prophylaxis of veterinary trypanosomiasis. A method for the simultaneous quantitation of the major constituents M&B4180A, 3-(3-m-amidinophenyl-2-triazeno)-8-amino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B38897), 7-(m-amidinophenyldiazo)-3,8-diamino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B4250) and 3,8-di(3-m-amidinophenyltriazeno)-5-ethyl-6-phenylphenanthridinium chloride dihydrochloride (M&B4596) is described. The related substances are resolved on a Gemini C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase composed of a mixture of acetonitrile and 50 mM ammonium formate buffer pH 2.8 (25:75 v/v) at a flow rate of 1 ml/min with UV detection at 320 nm. The method is compatible with electrospray ionisation mass spectrometry and provides a tool for the control of substandard and counterfeit commercial preparations of isometamidium.
| Original language | English |
|---|---|
| Pages (from-to) | 45-51 |
| Number of pages | 6 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 46 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 7 Jan 2008 |
Keywords
- isometamidium
- HPLC
- LC-MS
- counterfeit medicines
- related substances
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Cite this
Schad, G. J., Allanson, A., Mackay, S. P., Cannavan, A., & Tettey, J. N. A. (2008). Development and validation of an improved hplc method for the control of potentially counterfeit isometamidium products. Journal of Pharmaceutical and Biomedical Analysis, 46(1), 45-51. https://doi.org/10.1016/j.jpba.2007.08.026