Development and validation of an improved hplc method for the control of potentially counterfeit isometamidium products

G.J. Schad, A. Allanson, S.P. Mackay, A. Cannavan, J.N.A. Tettey

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Isometamidium, a mixture of related substances of which 8-(3-m-amidinophenyl-2-triazeno)-3-amino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B4180A) is the principal active component, is the only chemical agent available for prophylaxis of veterinary trypanosomiasis. A method for the simultaneous quantitation of the major constituents M&B4180A, 3-(3-m-amidinophenyl-2-triazeno)-8-amino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B38897), 7-(m-amidinophenyldiazo)-3,8-diamino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B4250) and 3,8-di(3-m-amidinophenyltriazeno)-5-ethyl-6-phenylphenanthridinium chloride dihydrochloride (M&B4596) is described. The related substances are resolved on a Gemini C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase composed of a mixture of acetonitrile and 50 mM ammonium formate buffer pH 2.8 (25:75 v/v) at a flow rate of 1 ml/min with UV detection at 320 nm. The method is compatible with electrospray ionisation mass spectrometry and provides a tool for the control of substandard and counterfeit commercial preparations of isometamidium.
Original languageEnglish
Pages (from-to)45-51
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume46
Issue number1
DOIs
Publication statusPublished - 7 Jan 2008

Keywords

  • isometamidium
  • HPLC
  • LC-MS
  • counterfeit medicines
  • related substances

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