Abstract
This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-coglycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.
Original language | English |
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Pages (from-to) | 268-278 |
Number of pages | 11 |
Journal | Current Drug Delivery |
Volume | 10 |
Issue number | 3 |
Publication status | Published - Jun 2013 |
Keywords
- adjuvants, immunologic
- antigen presentation
- Ag85B-ESAT-6
- microspheres
- poly(lactide-coglycolide)
- dimethyldioctadecylammonium bromide
- microsphere formulations