Developing solid particulate vaccine adjuvants: surface bound antigen favours a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

Daniel J. Kirby, Randip Kaur, Else M. Agger, Peter Andersen, Vincent W. Bramwell, Yvonne Perrie

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-coglycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

Original languageEnglish
Pages (from-to)268-278
Number of pages11
JournalCurrent Drug Delivery
Volume10
Issue number3
Publication statusPublished - Jun 2013

Keywords

  • adjuvants, immunologic
  • antigen presentation
  • Ag85B-ESAT-6
  • microspheres
  • poly(lactide-coglycolide)
  • dimethyldioctadecylammonium bromide
  • microsphere formulations

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