Developing solid particulate vaccine adjuvants: surface bound antigen favours a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

Daniel J. Kirby, Randip Kaur, Else M. Agger, Peter Andersen, Vincent W. Bramwell, Yvonne Perrie

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-coglycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

LanguageEnglish
Pages268-278
Number of pages11
JournalCurrent Drug Delivery
Volume10
Issue number3
Publication statusPublished - Jun 2013

Fingerprint

Surface Antigens
Cellular Immunity
Vaccines
Antigens
Microspheres
Emulsions
Antigen Presentation
Chitosan

Keywords

  • adjuvants, immunologic
  • antigen presentation
  • Ag85B-ESAT-6
  • microspheres
  • poly(lactide-coglycolide)
  • dimethyldioctadecylammonium bromide
  • microsphere formulations

Cite this

@article{2d2002f804e940e1960cca86efd5a00d,
title = "Developing solid particulate vaccine adjuvants: surface bound antigen favours a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity",
abstract = "This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-coglycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.",
keywords = "adjuvants, immunologic, antigen presentation, Ag85B-ESAT-6, microspheres , poly(lactide-coglycolide) , dimethyldioctadecylammonium bromide, microsphere formulations",
author = "Kirby, {Daniel J.} and Randip Kaur and Agger, {Else M.} and Peter Andersen and Bramwell, {Vincent W.} and Yvonne Perrie",
year = "2013",
month = "6",
language = "English",
volume = "10",
pages = "268--278",
journal = "Current Drug Delivery",
issn = "1567-2018",
number = "3",

}

Developing solid particulate vaccine adjuvants : surface bound antigen favours a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity. / Kirby, Daniel J.; Kaur, Randip; Agger, Else M.; Andersen, Peter; Bramwell, Vincent W.; Perrie, Yvonne.

In: Current Drug Delivery, Vol. 10, No. 3, 06.2013, p. 268-278.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Developing solid particulate vaccine adjuvants

T2 - Current Drug Delivery

AU - Kirby, Daniel J.

AU - Kaur, Randip

AU - Agger, Else M.

AU - Andersen, Peter

AU - Bramwell, Vincent W.

AU - Perrie, Yvonne

PY - 2013/6

Y1 - 2013/6

N2 - This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-coglycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

AB - This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-coglycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

KW - adjuvants, immunologic

KW - antigen presentation

KW - Ag85B-ESAT-6

KW - microspheres

KW - poly(lactide-coglycolide)

KW - dimethyldioctadecylammonium bromide

KW - microsphere formulations

M3 - Article

VL - 10

SP - 268

EP - 278

JO - Current Drug Delivery

JF - Current Drug Delivery

SN - 1567-2018

IS - 3

ER -