This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.
|Journal||Proceedings of SPIE - The International Society for Optical Engineering|
|Publication status||Published - 2006|
- time-resolved fret
- maximum entropy method
Rolinski, O. J., Hernandez-Santana, A., & Graham, D. (2006). Determining biomolecular structures by time-resolved fluorescence. Proceedings of SPIE - The International Society for Optical Engineering, 6047(60472N). https://doi.org/10.1117/12.710984