Determining biomolecular structures by time-resolved fluorescence

O.J. Rolinski, A. Hernandez-Santana, D. Graham

Research output: Contribution to journalArticle

Abstract

This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.
LanguageEnglish
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume6047
Issue number60472N
DOIs
Publication statusPublished - 2006

Fingerprint

Energy Transfer
resonance fluorescence
Fluorescence
Perylene
energy transfer
Bisbenzimidazole
fluorescence
Cobalt
Distribution functions
Distribution Function
DNA
cobalt
deoxyribonucleic acid
Kinetics
distribution functions
Ions
kinetics
Demonstrate
ions
Fluorescence Resonance Energy Transfer

Keywords

  • time-resolved fret
  • maximum entropy method
  • dsdna
  • perylene

Cite this

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abstract = "This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.",
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Determining biomolecular structures by time-resolved fluorescence. / Rolinski, O.J.; Hernandez-Santana, A.; Graham, D.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 6047, No. 60472N, 2006.

Research output: Contribution to journalArticle

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AU - Rolinski, O.J.

AU - Hernandez-Santana, A.

AU - Graham, D.

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KW - perylene

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