Determining biomolecular structures by time-resolved fluorescence

O.J. Rolinski; A. Hernandez-Santana; D. Graham

doi:10.1117/12.710984

Determining biomolecular structures by time-resolved fluorescence

O.J. Rolinski, A. Hernandez-Santana, D. Graham

Research output: Contribution to journal › Article › peer-review

Abstract

This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

Original language	English
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6047
Issue number	60472N
DOIs	https://doi.org/10.1117/12.710984
Publication status	Published - 2006

Keywords

time-resolved fret
maximum entropy method
dsdna
perylene

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10.1117/12.710984

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title = "Determining biomolecular structures by time-resolved fluorescence",

abstract = "This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.",

keywords = "time-resolved fret, maximum entropy method, dsdna, perylene",

author = "O.J. Rolinski and A. Hernandez-Santana and D. Graham",

note = "Fourth International Conference on Photonics and Imaging in Biology and Medicine. Subseries of Proceedings of SPIE - International Society for Optical Engineering. Vol. 6047, Part 2. Progress in Biomedical Optics and Imaging, Vol. 7(2) Subseries of Proceedings of SPIE.",

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AU - Rolinski, O.J.

AU - Hernandez-Santana, A.

AU - Graham, D.

N1 - Fourth International Conference on Photonics and Imaging in Biology and Medicine. Subseries of Proceedings of SPIE - International Society for Optical Engineering. Vol. 6047, Part 2. Progress in Biomedical Optics and Imaging, Vol. 7(2) Subseries of Proceedings of SPIE.

PY - 2006

Y1 - 2006

N2 - This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

AB - This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

KW - time-resolved fret

KW - maximum entropy method

KW - dsdna

KW - perylene

UR - http://direct.bl.uk/bld/PlaceOrder.do?UIN=196776106&ETOC=RN&from=searchengine

UR - http://spiedl.aip.org/journals/doc/SPIEDL-home/proc/

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VL - 6047

JO - Proceedings of SPIE

JF - Proceedings of SPIE

SN - 0277-786X

IS - 60472N

ER -

Determining biomolecular structures by time-resolved fluorescence

Abstract

Keywords

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