Determining biomolecular structures by time-resolved fluorescence

O.J. Rolinski, A. Hernandez-Santana, D. Graham

Research output: Contribution to journalArticle

Abstract

This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.
Original languageEnglish
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume6047
Issue number60472N
DOIs
Publication statusPublished - 2006

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Keywords

  • time-resolved fret
  • maximum entropy method
  • dsdna
  • perylene

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