Abstract
| Language | English |
|---|---|
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 6047 |
| Issue number | 60472N |
| DOIs | |
| Publication status | Published - 2006 |
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Keywords
- time-resolved fret
- maximum entropy method
- dsdna
- perylene
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Determining biomolecular structures by time-resolved fluorescence. / Rolinski, O.J.; Hernandez-Santana, A.; Graham, D.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 6047, No. 60472N, 2006.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determining biomolecular structures by time-resolved fluorescence
AU - Rolinski, O.J.
AU - Hernandez-Santana, A.
AU - Graham, D.
N1 - Fourth International Conference on Photonics and Imaging in Biology and Medicine. Subseries of Proceedings of SPIE - International Society for Optical Engineering. Vol. 6047, Part 2. Progress in Biomedical Optics and Imaging, Vol. 7(2) Subseries of Proceedings of SPIE.
PY - 2006
Y1 - 2006
N2 - This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.
AB - This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.
KW - time-resolved fret
KW - maximum entropy method
KW - dsdna
KW - perylene
UR - http://direct.bl.uk/bld/PlaceOrder.do?UIN=196776106&ETOC=RN&from=searchengine
UR - http://spiedl.aip.org/journals/doc/SPIEDL-home/proc/
UR - http://dx.doi.org/10.1117/12.710984
U2 - 10.1117/12.710984
DO - 10.1117/12.710984
M3 - Article
VL - 6047
JO - Proceedings of SPIE
T2 - Proceedings of SPIE
JF - Proceedings of SPIE
SN - 0277-786X
IS - 60472N
ER -