Determining biomolecular structures by time-resolved fluorescence

O.J. Rolinski; A. Hernandez-Santana; D. Graham

doi:10.1117/12.710984

Determining biomolecular structures by time-resolved fluorescence

O.J. Rolinski, A. Hernandez-Santana, D. Graham

Research output: Contribution to journal › Article

Abstract

This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

Original language	English
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6047
Issue number	60472N
DOIs	https://doi.org/10.1117/12.710984
Publication status	Published - 2006

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Keywords

time-resolved fret
maximum entropy method
dsdna
perylene

Cite this

Rolinski, O. J., Hernandez-Santana, A., & Graham, D. (2006). Determining biomolecular structures by time-resolved fluorescence. Proceedings of SPIE - The International Society for Optical Engineering, 6047(60472N). https://doi.org/10.1117/12.710984

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AU - Hernandez-Santana, A.

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AB - This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

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KW - maximum entropy method

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KW - perylene

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10.1117/12.710984