Determination of the potential bioavailability of plant microRNAs using a simulated human digestion process

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Abstract

The “dietary xenomiR hypothesis” proposes that microRNAs (miRNAs) in foodstuffs survive transit through the mammalian gastrointestinal tract and pass into cells intact to affect gene regulation. However, debate continues as to whether dietary intake poses a feasible route for such exogenous gene regulators. Understanding on miRNA levels during pre-treatments of human diet is essential to test their bioavailability during digestion. This study makes the novel first use of an in vitro method to eliminate the inherent complexities and variability of in vivo approaches used to test this hypothesis. Plant miRNA levels in soybean and rice were measured during storage, processing, cooking, and early digestion using real-time PCR. We have demonstrated for the first time that storage, processing, and cooking does not abolish the plant miRNAs present in the foodstuffs. In addition, utilizing a simulated human digestion system revealed significant plant miRNA bioavailability after early stage digestion for 75 min. Attenuation of plant messenger RNA and synthetic miRNA was observed under these conditions. Even after an extensive pretreatment, plant-derived miRNA, delivered by typical dietary ingestion, has a robustness that could make them bioavailable for uptake during early digestion. The potential benefit of these regulatory molecules in pharma-nutrition could be explored further.
LanguageEnglish
JournalMolecular Nutrition and Food Research
Early online date6 Jul 2015
DOIs
Publication statusPublished - 2015

Fingerprint

MicroRNAs
microRNA
Biological Availability
bioavailability
Digestion
digestion
Cooking
cooking
pretreatment
Plant RNA
Regulator Genes
messenger RNA
regulator genes
Soybeans
gastrointestinal system
Gastrointestinal Tract
Real-Time Polymerase Chain Reaction
food intake
storage time
quantitative polymerase chain reaction

Keywords

  • cross kingdom regulation
  • simulated digestion
  • nutraceutical
  • MicroRNA
  • diet

Cite this

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title = "Determination of the potential bioavailability of plant microRNAs using a simulated human digestion process",
abstract = "The “dietary xenomiR hypothesis” proposes that microRNAs (miRNAs) in foodstuffs survive transit through the mammalian gastrointestinal tract and pass into cells intact to affect gene regulation. However, debate continues as to whether dietary intake poses a feasible route for such exogenous gene regulators. Understanding on miRNA levels during pre-treatments of human diet is essential to test their bioavailability during digestion. This study makes the novel first use of an in vitro method to eliminate the inherent complexities and variability of in vivo approaches used to test this hypothesis. Plant miRNA levels in soybean and rice were measured during storage, processing, cooking, and early digestion using real-time PCR. We have demonstrated for the first time that storage, processing, and cooking does not abolish the plant miRNAs present in the foodstuffs. In addition, utilizing a simulated human digestion system revealed significant plant miRNA bioavailability after early stage digestion for 75 min. Attenuation of plant messenger RNA and synthetic miRNA was observed under these conditions. Even after an extensive pretreatment, plant-derived miRNA, delivered by typical dietary ingestion, has a robustness that could make them bioavailable for uptake during early digestion. The potential benefit of these regulatory molecules in pharma-nutrition could be explored further.",
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