Determination of an optimal axial-length tension for the study of isolated resistance arteries on a pressure myograph

P Coats, C Hillier

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Abstract

The effects of longitudinal stretch on vasoreactivity were assessed in isolated, pressurised arteries from the rat mesentery, rat mid-cerebral and human subcutaneous vascular beds. A stretch-dependent increase in reactivity was observed only in rat third order mesenteric arteries. Longitudinal stretch > 20 % (force equal to 0.23 +/- 0.04 mN) optimises vasoconstrictor responses to noradrenaline and phenylephrine in rat third order mesenteric arteries. Stretch did not affect the vasoconstriction response to depolarisation by 30 mM K+ PSS in any of the arteries studied. Similarly, stretch had no affect on pressure-dependent myogenic responses in rat mid-cerebral arteries. Endothelium-independent and endothelium-dependent mechanisms of vasorelaxation were unaffected by stretch in rat third order mesenteric arteries. Likewise, stretch did not affect vasoreactivity in rat mid-cerebral and human subcutaneous resistance arteries. Our results show that longitudinal stretch in isobaric-mounted rat third order mesenteric arteries is an important methodological consideration. Considering our results, we recommend that isobaric-mounted rat third order mesenteric arteries are stretched > 20 % to provide optimal experimental conditions for pharmacological studies.

Original languageEnglish
Pages (from-to)1085–1094
Number of pages10
JournalExperimental Physiology
Volume84
Issue number6
DOIs
Publication statusPublished - Nov 1999

Keywords

  • animals
  • cerebral arteries
  • humans
  • male
  • mesenteric arteries
  • muscle contraction
  • muscle, smooth, vascular
  • myography
  • norepinephrine
  • phenylephrine
  • potassium
  • rats
  • rats, wistar
  • vasoconstriction
  • vasoconstrictor agents
  • vasodilation

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