Determination of acceptor distribution from fluorescence resonance energy transfer: theory and simulation

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Abstract

A new method for determining the donor-acceptor distribution function in fluorescence resonance energy transfer (FRET) systems is presented. The approach is based on time-resolved fluorescence experiments with nanosecond resolution. Potential applications of this method include: determination of the morphology of porous materials (e.g., polymers, sol-gels, resins, etc.), monitoring processes occurring on the nanometer scale including biomolecules labeled with the donor/acceptor species, and FRET sensors based on competitive binding. In this paper a theoretical derivation of the method is presented and the method is tested in a series of numerical simulations. The experimental conditions regarding this approach are discussed and its applicability to real measurement systems is demonstrated
Original languageEnglish
Pages (from-to)8923-8933
Number of pages10
JournalJournal of Chemical Physics
Volume112
Issue number20
DOIs
Publication statusPublished - 22 May 2000

Keywords

  • FRET
  • fluorescence resonance energy transfer

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