Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres

Matteo Fallani, Sergio Amarri, Agneta Uusijarvi, Rüdiger Adam, Sheila Khanna, Marga Aguilera, Angel Gil, Jose M Vieites, Elisabeth Norin, David Young, Jane A Scott, Joël Doré, Christine A Edwards, the INFABIO team

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Although it is well established that early infant feeding has a major influence on the establishment of the gut microbiota, very little is understood about how the introduction of first solid food influences the colonization process. This study aimed to determine the impact of weaning on the faecal microbiota composition of infants from five European countries (Sweden, Scotland, Germany, Italy and Spain) which have different lifestyle characteristics and infant feeding practices. Faecal samples were collected from 605 infants approximately 4 weeks after the introduction of first solid foods and the results were compared with the same infants before weaning (6 weeks of age) to investigate the association with determining factors such as geographical origin, mode of delivery, previous feeding method and age of weaning. Samples were analysed by fluorescence in situ hybridization and flow cytometry using a panel of 10 rRNA targeted group- and species-specific oligonucleotide probes. The genus Bifidobacterium (36.5 % average proportion of total detectable bacteria), Clostridium coccoides group (14 %) and Bacteroides (13.6 %) were predominant after weaning. Similar to pre-weaning, northern European countries were associated with a higher proportion of bifidobacteria in the infant gut microbiota while higher levels of Bacteroides and lactobacilli characterized southern European countries. As before weaning, the initial feeding method influenced the Clostridium leptum group and Clostridium difficile+Clostridium perfringens species, and bifidobacteria still dominated the faeces of initially breast-fed infants. Formula-fed babies presented significantly higher proportions of Bacteroides and the C. coccoides group. The mode of birth influenced changes in the proportions of bacteroides and atopobium. Although there were significant differences in the mean weaning age between countries, this was not related to the populations of bifidobacteria or bacteroides. Thus, although the faecal microbiota of infants after first complementary foods was different to that before weaning commenced, many of the initial influences on microbiota composition were still evident.
LanguageEnglish
Pages1385-1392
Number of pages8
JournalMicrobiology
Volume157
Issue numberPt 5
Early online date17 Feb 2011
DOIs
Publication statusPublished - 2011

Fingerprint

Infant Food
Weaning
Bacteroides
Bifidobacterium
Microbiota
Feeding Methods
Clostridium
Food
Infant Formula
Clostridium perfringens
Clostridium difficile
Oligonucleotide Probes
Gastrointestinal Microbiome
Scotland
Lactobacillus
Fluorescence In Situ Hybridization
Sweden
Feces
Spain
Italy

Keywords

  • FISH
  • , fluorescence in situ hybridization
  • infant feeding
  • gut microbiota
  • solid food

Cite this

Fallani, Matteo ; Amarri, Sergio ; Uusijarvi, Agneta ; Adam, Rüdiger ; Khanna, Sheila ; Aguilera, Marga ; Gil, Angel ; Vieites, Jose M ; Norin, Elisabeth ; Young, David ; Scott, Jane A ; Doré, Joël ; Edwards, Christine A ; the INFABIO team. / Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres. In: Microbiology. 2011 ; Vol. 157, No. Pt 5. pp. 1385-1392.
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abstract = "Although it is well established that early infant feeding has a major influence on the establishment of the gut microbiota, very little is understood about how the introduction of first solid food influences the colonization process. This study aimed to determine the impact of weaning on the faecal microbiota composition of infants from five European countries (Sweden, Scotland, Germany, Italy and Spain) which have different lifestyle characteristics and infant feeding practices. Faecal samples were collected from 605 infants approximately 4 weeks after the introduction of first solid foods and the results were compared with the same infants before weaning (6 weeks of age) to investigate the association with determining factors such as geographical origin, mode of delivery, previous feeding method and age of weaning. Samples were analysed by fluorescence in situ hybridization and flow cytometry using a panel of 10 rRNA targeted group- and species-specific oligonucleotide probes. The genus Bifidobacterium (36.5 {\%} average proportion of total detectable bacteria), Clostridium coccoides group (14 {\%}) and Bacteroides (13.6 {\%}) were predominant after weaning. Similar to pre-weaning, northern European countries were associated with a higher proportion of bifidobacteria in the infant gut microbiota while higher levels of Bacteroides and lactobacilli characterized southern European countries. As before weaning, the initial feeding method influenced the Clostridium leptum group and Clostridium difficile+Clostridium perfringens species, and bifidobacteria still dominated the faeces of initially breast-fed infants. Formula-fed babies presented significantly higher proportions of Bacteroides and the C. coccoides group. The mode of birth influenced changes in the proportions of bacteroides and atopobium. Although there were significant differences in the mean weaning age between countries, this was not related to the populations of bifidobacteria or bacteroides. Thus, although the faecal microbiota of infants after first complementary foods was different to that before weaning commenced, many of the initial influences on microbiota composition were still evident.",
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author = "Matteo Fallani and Sergio Amarri and Agneta Uusijarvi and R{\"u}diger Adam and Sheila Khanna and Marga Aguilera and Angel Gil and Vieites, {Jose M} and Elisabeth Norin and David Young and Scott, {Jane A} and Jo{\"e}l Dor{\'e} and Edwards, {Christine A} and {the INFABIO team}",
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Fallani, M, Amarri, S, Uusijarvi, A, Adam, R, Khanna, S, Aguilera, M, Gil, A, Vieites, JM, Norin, E, Young, D, Scott, JA, Doré, J, Edwards, CA & the INFABIO team 2011, 'Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres' Microbiology, vol. 157, no. Pt 5, pp. 1385-1392. https://doi.org/10.1099/mic.0.042143-0

Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres. / Fallani, Matteo; Amarri, Sergio; Uusijarvi, Agneta; Adam, Rüdiger; Khanna, Sheila; Aguilera, Marga; Gil, Angel; Vieites, Jose M; Norin, Elisabeth; Young, David; Scott, Jane A; Doré, Joël; Edwards, Christine A; the INFABIO team.

In: Microbiology, Vol. 157, No. Pt 5, 2011, p. 1385-1392.

Research output: Contribution to journalArticle

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AU - Adam, Rüdiger

AU - Khanna, Sheila

AU - Aguilera, Marga

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