Determinants of synonymous and nonsynonymous variability in three species of Drosophila

Penelope R Haddrill, Kai Zeng, Brian Charlesworth

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We estimated the intensity of selection on preferred codons in Drosophila pseudoobscura and D. miranda at X-linked and autosomal loci, using a published data set on sequence variability at 67 loci, by means of an improved method that takes account of demographic effects. We found evidence for stronger selection at X-linked loci, consistent with their higher levels of codon usage bias. The estimates of the strength of selection and mutational bias in favor of unpreferred codons were similar to those found in other species, after taking into account the fact that D. pseudoobscura showed evidence for a recent expansion in population size. We examined correlates of synonymous and nonsynonymous diversity in these species and found no evidence for effects of recurrent selective sweeps on nonsynonymous mutations, which is probably because this set of genes have much higher than average levels of selective constraints. There was evidence for correlated effects of levels of selective constraints on protein sequences and on codon usage, as expected under models of selection for translational accuracy. Our analysis of a published data set on D. melanogaster provided evidence for the effects of selective sweeps of nonsynonymous mutations on linked synonymous diversity, but only in the subset of loci that experienced the highest rates of nonsynonymous substitutions (about one-quarter of the total) and not at more slowly evolving loci. Our correlational analysis of this data set suggested that both selective constraints on protein sequences and recurrent selective sweeps affect the overall level of codon usage.

Original languageEnglish
Pages (from-to)1731-1743
Number of pages13
JournalMolecular Biology and Evolution
Volume28
Issue number5
Early online date29 Dec 2010
DOIs
Publication statusPublished - May 2011

Fingerprint

codons
Codon
Drosophila
loci
Drosophila pseudoobscura
mutation
Proteins
Substitution reactions
Genes
data analysis
amino acid sequences
protein
Mutation
Melanogaster
selection intensity
Selection Bias
population size
Population Density
substitution
demographic statistics

Keywords

  • algorithms
  • animals
  • codon
  • Computer simulation
  • drosophila
  • evolution, molecular
  • gene expression profiling
  • genes, x-linked
  • genetic variation
  • likelihood functions
  • models, genetic
  • polymorphism, genetic
  • population growth
  • selection, genetic

Cite this

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title = "Determinants of synonymous and nonsynonymous variability in three species of Drosophila",
abstract = "We estimated the intensity of selection on preferred codons in Drosophila pseudoobscura and D. miranda at X-linked and autosomal loci, using a published data set on sequence variability at 67 loci, by means of an improved method that takes account of demographic effects. We found evidence for stronger selection at X-linked loci, consistent with their higher levels of codon usage bias. The estimates of the strength of selection and mutational bias in favor of unpreferred codons were similar to those found in other species, after taking into account the fact that D. pseudoobscura showed evidence for a recent expansion in population size. We examined correlates of synonymous and nonsynonymous diversity in these species and found no evidence for effects of recurrent selective sweeps on nonsynonymous mutations, which is probably because this set of genes have much higher than average levels of selective constraints. There was evidence for correlated effects of levels of selective constraints on protein sequences and on codon usage, as expected under models of selection for translational accuracy. Our analysis of a published data set on D. melanogaster provided evidence for the effects of selective sweeps of nonsynonymous mutations on linked synonymous diversity, but only in the subset of loci that experienced the highest rates of nonsynonymous substitutions (about one-quarter of the total) and not at more slowly evolving loci. Our correlational analysis of this data set suggested that both selective constraints on protein sequences and recurrent selective sweeps affect the overall level of codon usage.",
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Determinants of synonymous and nonsynonymous variability in three species of Drosophila. / Haddrill, Penelope R; Zeng, Kai; Charlesworth, Brian.

In: Molecular Biology and Evolution, Vol. 28, No. 5, 05.2011, p. 1731-1743.

Research output: Contribution to journalArticle

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