Detection of inflammation in vivo by surface-enhanced Raman scattering provides higher sensitivity than conventional fluorescence imaging

Ross McQueenie, Ross Stevenson, Robert Benson, Neil MacRitchie, Iain McInnes, Pasquale Maffia, Karen Faulds, Duncan Graham, James Brewer, Paul Garside

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The detection of inflammatory changes is a key aim for the early diagnosis and treatment of several autoimmune, infectious, and metastatic diseases. While surface-enhanced Raman scattering (SERS) has the capability to provide noninvasive, in vivo imaging at sufficient depth to achieve this goal, this approach has not been exploited in the study of inflammation. SERS-active nanoparticles were coded with a unique Raman signal that was protected under a wide range of conditions and stimuli. To detect early-stage inflammation, gold nanoparticle clusters containing Raman-active molecules were conjugated to intercellular adhesion molecule 1- (ICAM-1-) specific monoclonal antibodies. SERS allowed noninvasive measurement of ICAM-1 expression in vivo with twice the sensitivity of two-photon fluorescence. This is the first time SERS has been used for in vivo detection of inflammation and is a major advance in the ever-growing toolkit of approaches for use in noninvasive, next-generation in vivo imaging.

Original languageEnglish
Pages (from-to)5968-5975
Number of pages8
JournalAnalytical Chemistry
Volume84
Issue number14
DOIs
Publication statusPublished - 17 Jul 2012

Keywords

  • fluorescence imaging
  • Raman scattering
  • inflammation
  • autoimmune diseases

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