Detection of dna and p-450s on silver colloidal nanoparticles by surface-enhanced resonance raman scattering (SERRS)

W.E. Smith, E. Polwart, C. McLaughlin, B.J. Mallinder, S.J. Smith, D. Graham

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Surface-enhanced resonance Raman scattering (SERRS) is a very sensitive and selective detection method that can be used for the analysis of both DNA and P-450s. A number of factors have limited the broader application of the technique. These limitations are described and addressed. An approach to reduce the problems associated with variation of the silver colloids used to provide surface enhancement and chemical methodologies that ensure surface adsorption are presented. A practical approach was used to investigate the nature of the effect. This approach has highlighted the importance of resonance enhancement for ultimate sensitivity. Two approaches to achieve successful detection of DNA using SERRS are described, and, using these two approaches, the possibility of multiplexing is also demonstrated. The analysis of proteins by SERRS is discussed and P-450 is presented as a specific example of the information that may be gained from SERRS of proteins.
LanguageEnglish
Title of host publicationProceedings of SPIE: Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications
Pages73-79
Number of pages6
Volume4258
DOIs
Publication statusPublished - 2001

Fingerprint

Raman scattering
Nanoparticles
DNA
Multiplexing
Membrane Proteins
colloidal silver
Adsorption
Proteins

Keywords

  • surface-enhanced raman scattering (sers)
  • surface-enhanced resonance raman scattering
  • silver colloid
  • dna

Cite this

Smith, W. E., Polwart, E., McLaughlin, C., Mallinder, B. J., Smith, S. J., & Graham, D. (2001). Detection of dna and p-450s on silver colloidal nanoparticles by surface-enhanced resonance raman scattering (SERRS). In Proceedings of SPIE: Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications (Vol. 4258, pp. 73-79) https://doi.org/10.1117/12.430763
Smith, W.E. ; Polwart, E. ; McLaughlin, C. ; Mallinder, B.J. ; Smith, S.J. ; Graham, D. / Detection of dna and p-450s on silver colloidal nanoparticles by surface-enhanced resonance raman scattering (SERRS). Proceedings of SPIE: Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications. Vol. 4258 2001. pp. 73-79
@inbook{418e4487b305494d8490d7ffcf2ca8ca,
title = "Detection of dna and p-450s on silver colloidal nanoparticles by surface-enhanced resonance raman scattering (SERRS)",
abstract = "Surface-enhanced resonance Raman scattering (SERRS) is a very sensitive and selective detection method that can be used for the analysis of both DNA and P-450s. A number of factors have limited the broader application of the technique. These limitations are described and addressed. An approach to reduce the problems associated with variation of the silver colloids used to provide surface enhancement and chemical methodologies that ensure surface adsorption are presented. A practical approach was used to investigate the nature of the effect. This approach has highlighted the importance of resonance enhancement for ultimate sensitivity. Two approaches to achieve successful detection of DNA using SERRS are described, and, using these two approaches, the possibility of multiplexing is also demonstrated. The analysis of proteins by SERRS is discussed and P-450 is presented as a specific example of the information that may be gained from SERRS of proteins.",
keywords = "surface-enhanced raman scattering (sers), surface-enhanced resonance raman scattering, silver colloid, dna",
author = "W.E. Smith and E. Polwart and C. McLaughlin and B.J. Mallinder and S.J. Smith and D. Graham",
year = "2001",
doi = "10.1117/12.430763",
language = "English",
volume = "4258",
pages = "73--79",
booktitle = "Proceedings of SPIE: Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications",

}

Smith, WE, Polwart, E, McLaughlin, C, Mallinder, BJ, Smith, SJ & Graham, D 2001, Detection of dna and p-450s on silver colloidal nanoparticles by surface-enhanced resonance raman scattering (SERRS). in Proceedings of SPIE: Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications. vol. 4258, pp. 73-79. https://doi.org/10.1117/12.430763

Detection of dna and p-450s on silver colloidal nanoparticles by surface-enhanced resonance raman scattering (SERRS). / Smith, W.E.; Polwart, E.; McLaughlin, C.; Mallinder, B.J.; Smith, S.J.; Graham, D.

Proceedings of SPIE: Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications. Vol. 4258 2001. p. 73-79.

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Detection of dna and p-450s on silver colloidal nanoparticles by surface-enhanced resonance raman scattering (SERRS)

AU - Smith, W.E.

AU - Polwart, E.

AU - McLaughlin, C.

AU - Mallinder, B.J.

AU - Smith, S.J.

AU - Graham, D.

PY - 2001

Y1 - 2001

N2 - Surface-enhanced resonance Raman scattering (SERRS) is a very sensitive and selective detection method that can be used for the analysis of both DNA and P-450s. A number of factors have limited the broader application of the technique. These limitations are described and addressed. An approach to reduce the problems associated with variation of the silver colloids used to provide surface enhancement and chemical methodologies that ensure surface adsorption are presented. A practical approach was used to investigate the nature of the effect. This approach has highlighted the importance of resonance enhancement for ultimate sensitivity. Two approaches to achieve successful detection of DNA using SERRS are described, and, using these two approaches, the possibility of multiplexing is also demonstrated. The analysis of proteins by SERRS is discussed and P-450 is presented as a specific example of the information that may be gained from SERRS of proteins.

AB - Surface-enhanced resonance Raman scattering (SERRS) is a very sensitive and selective detection method that can be used for the analysis of both DNA and P-450s. A number of factors have limited the broader application of the technique. These limitations are described and addressed. An approach to reduce the problems associated with variation of the silver colloids used to provide surface enhancement and chemical methodologies that ensure surface adsorption are presented. A practical approach was used to investigate the nature of the effect. This approach has highlighted the importance of resonance enhancement for ultimate sensitivity. Two approaches to achieve successful detection of DNA using SERRS are described, and, using these two approaches, the possibility of multiplexing is also demonstrated. The analysis of proteins by SERRS is discussed and P-450 is presented as a specific example of the information that may be gained from SERRS of proteins.

KW - surface-enhanced raman scattering (sers)

KW - surface-enhanced resonance raman scattering

KW - silver colloid

KW - dna

UR - http://adsabs.harvard.edu/abs/2001SPIE.4258...73S

UR - http://dx.doi.org/10.1117/12.430763

U2 - 10.1117/12.430763

DO - 10.1117/12.430763

M3 - Chapter

VL - 4258

SP - 73

EP - 79

BT - Proceedings of SPIE: Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications

ER -

Smith WE, Polwart E, McLaughlin C, Mallinder BJ, Smith SJ, Graham D. Detection of dna and p-450s on silver colloidal nanoparticles by surface-enhanced resonance raman scattering (SERRS). In Proceedings of SPIE: Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications. Vol. 4258. 2001. p. 73-79 https://doi.org/10.1117/12.430763