Detection of cortisol in serum using quantitative resonance Raman spectroscopy

Kirsten Gracie, Susan Pang, Gerwyn M. Jones, Karen Faulds, Julian Braybrook, Duncan Graham*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)
56 Downloads (Pure)


Measurement of cortisol in serum is used commonly as an indicator of stress and disease. Conventional analytical techniques have limited utility given that they remain largely laboratory based, they do not directly measure the deemed biologically active free cortisol, and there is no robust correlation between the free cortisol measurements within serum and saliva. It would therefore be desirable to measure both the free and total cortisol readily within the same matrix in a portable device in the field or at the bedside. This paper demonstrates the utility of a portable Raman approach to measure both the biological active free cortisol as well as total cortisol in human serum, compared to a laboratory-based chemiluminescence analysis technique. This alternative portable Raman method produced results that were consistent with results obtained from previous methods, which has the potential for further miniaturisation for point of test applications.
Original languageEnglish
Pages (from-to)1589-1594
Number of pages6
JournalAnalytical Methods
Issue number10
Early online date13 Feb 2017
Publication statusPublished - 14 Mar 2017


  • corticol
  • serum
  • free cortisol measurements
  • quantitative resonance raman spectroscopy
  • portable device
  • resonance raman scattering
  • stress monitoring


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