Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe

Duncan Graham, W. Ewen Smith, Adrian M. T. Linacre, Nigel D. Watson, Peter C. White, Bjørnar Olaisen (Editor), Bernd Brinkmann (Editor), Patrick J. Lincoln (Editor)

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The technique we are reporting, Surface Enhanced Resonance Raman Scattering, SERRS, exploits the light scattering, or Raman scattering, produced when a light beam illuminates molecules. The effect is weak and depends on the molecular structure so that some molecules are weaker scatters than others are. Accordingly signals from strong scatters can be detected in the presence of weaker ones such as water. The effect is enhanced up to 106 fold if the analyte is adsorbed onto a roughened metal surface, i.e. Surface Enhanced Resonance Scattering (SERS). A further enhancement is achieved by utilizing a dye as the analyte and by tuning the laser excitation frequency to the maximum of the dye chromophore to obtain resonance scattering from the surface i.e. SERRS.
LanguageEnglish
Title of host publicationProgress in Forensic Genetics 7: Proceedings of the 17th International ISFH Congress
Pages6-8
Number of pages3
Edition1167
Publication statusPublished - 1998

Publication series

NameInternational Congress Series
PublisherElsevier

Fingerprint

DNA sequences
Raman scattering
Coloring Agents
Scattering
Molecules
Laser excitation
Chromophores
Light scattering
Molecular structure
Tuning
Metals
Water

Keywords

  • dna sequence
  • dna
  • surface enhanced resonance
  • raman scattering
  • dna probe

Cite this

Graham, D., Smith, W. E., Linacre, A. M. T., Watson, N. D., White, P. C., Olaisen, B. (Ed.), ... Lincoln, P. J. (Ed.) (1998). Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe. In Progress in Forensic Genetics 7: Proceedings of the 17th International ISFH Congress (1167 ed., pp. 6-8). (International Congress Series).
Graham, Duncan ; Smith, W. Ewen ; Linacre, Adrian M. T. ; Watson, Nigel D. ; White, Peter C. ; Olaisen, Bjørnar (Editor) ; Brinkmann, Bernd (Editor) ; Lincoln, Patrick J. (Editor). / Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe. Progress in Forensic Genetics 7: Proceedings of the 17th International ISFH Congress. 1167. ed. 1998. pp. 6-8 (International Congress Series).
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abstract = "The technique we are reporting, Surface Enhanced Resonance Raman Scattering, SERRS, exploits the light scattering, or Raman scattering, produced when a light beam illuminates molecules. The effect is weak and depends on the molecular structure so that some molecules are weaker scatters than others are. Accordingly signals from strong scatters can be detected in the presence of weaker ones such as water. The effect is enhanced up to 106 fold if the analyte is adsorbed onto a roughened metal surface, i.e. Surface Enhanced Resonance Scattering (SERS). A further enhancement is achieved by utilizing a dye as the analyte and by tuning the laser excitation frequency to the maximum of the dye chromophore to obtain resonance scattering from the surface i.e. SERRS.",
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Graham, D, Smith, WE, Linacre, AMT, Watson, ND, White, PC, Olaisen, B (ed.), Brinkmann, B (ed.) & Lincoln, PJ (ed.) 1998, Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe. in Progress in Forensic Genetics 7: Proceedings of the 17th International ISFH Congress. 1167 edn, International Congress Series, pp. 6-8.

Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe. / Graham, Duncan; Smith, W. Ewen; Linacre, Adrian M. T.; Watson, Nigel D.; White, Peter C.; Olaisen, Bjørnar (Editor); Brinkmann, Bernd (Editor); Lincoln, Patrick J. (Editor).

Progress in Forensic Genetics 7: Proceedings of the 17th International ISFH Congress. 1167. ed. 1998. p. 6-8 (International Congress Series).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe

AU - Graham, Duncan

AU - Smith, W. Ewen

AU - Linacre, Adrian M. T.

AU - Watson, Nigel D.

AU - White, Peter C.

A2 - Olaisen, Bjørnar

A2 - Brinkmann, Bernd

A2 - Lincoln, Patrick J.

PY - 1998

Y1 - 1998

N2 - The technique we are reporting, Surface Enhanced Resonance Raman Scattering, SERRS, exploits the light scattering, or Raman scattering, produced when a light beam illuminates molecules. The effect is weak and depends on the molecular structure so that some molecules are weaker scatters than others are. Accordingly signals from strong scatters can be detected in the presence of weaker ones such as water. The effect is enhanced up to 106 fold if the analyte is adsorbed onto a roughened metal surface, i.e. Surface Enhanced Resonance Scattering (SERS). A further enhancement is achieved by utilizing a dye as the analyte and by tuning the laser excitation frequency to the maximum of the dye chromophore to obtain resonance scattering from the surface i.e. SERRS.

AB - The technique we are reporting, Surface Enhanced Resonance Raman Scattering, SERRS, exploits the light scattering, or Raman scattering, produced when a light beam illuminates molecules. The effect is weak and depends on the molecular structure so that some molecules are weaker scatters than others are. Accordingly signals from strong scatters can be detected in the presence of weaker ones such as water. The effect is enhanced up to 106 fold if the analyte is adsorbed onto a roughened metal surface, i.e. Surface Enhanced Resonance Scattering (SERS). A further enhancement is achieved by utilizing a dye as the analyte and by tuning the laser excitation frequency to the maximum of the dye chromophore to obtain resonance scattering from the surface i.e. SERRS.

KW - dna sequence

KW - dna

KW - surface enhanced resonance

KW - raman scattering

KW - dna probe

UR - http://dx.doi.org/10.1039/b500248f

M3 - Chapter

SN - 0444829652

T3 - International Congress Series

SP - 6

EP - 8

BT - Progress in Forensic Genetics 7: Proceedings of the 17th International ISFH Congress

ER -

Graham D, Smith WE, Linacre AMT, Watson ND, White PC, Olaisen B, (ed.) et al. Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe. In Progress in Forensic Genetics 7: Proceedings of the 17th International ISFH Congress. 1167 ed. 1998. p. 6-8. (International Congress Series).