Detection and visualization improvement of spermatozoa cells by digital holography

L. Miccio; A. Finizio; P. Memmolo; M. Paturzo; F. Merola; G. Coppola; G. Di Caprio; M. Gioffrè; R. Puglisi; D. Balduzzi; A. Galli; P. Ferraro

doi:10.1117/12.889972

Detection and visualization improvement of spermatozoa cells by digital holography

L. Miccio, A. Finizio, P. Memmolo, M. Paturzo, F. Merola, G. Coppola, G. Di Caprio, M. Gioffrè, R. Puglisi, D. Balduzzi, A. Galli, P. Ferraro

Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

7 Citations (Scopus)

Abstract

Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it's able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.

Original language	English
Title of host publication	Molecular Imaging III
Editors	Charles P. Lin, Vasilis Ntziachristos
Place of Publication	Bellingham, Washington
Number of pages	6
DOIs	https://doi.org/10.1117/12.889972
Publication status	Published - 14 Jun 2011
Event	European Conference on Biomedical Optics, ECBO 2011 - Munich, Germany Duration: 22 May 2011 → 26 May 2011

Publication series

Name	Proceedings of SPIE
Publisher	SPIE
Volume	8089
ISSN (Print)	1605-7422

Conference

Conference	European Conference on Biomedical Optics, ECBO 2011
Country/Territory	Germany
City	Munich
Period	22/05/11 → 26/05/11

Keywords

digital holography
image enhancement
interference microscopy

Access to Document

10.1117/12.889972

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Miccio, L., Finizio, A., Memmolo, P., Paturzo, M., Merola, F., Coppola, G., Di Caprio, G., Gioffrè, M., Puglisi, R., Balduzzi, D., Galli, A., & Ferraro, P. (2011). Detection and visualization improvement of spermatozoa cells by digital holography. In C. P. Lin, & V. Ntziachristos (Eds.), Molecular Imaging III Article 80890C (Proceedings of SPIE; Vol. 8089).. https://doi.org/10.1117/12.889972

@inproceedings{7a01868603f84f91b167406991036254,

title = "Detection and visualization improvement of spermatozoa cells by digital holography",

abstract = "Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it's able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.",

keywords = "digital holography, image enhancement, interference microscopy",

author = "L. Miccio and A. Finizio and P. Memmolo and M. Paturzo and F. Merola and G. Coppola and {Di Caprio}, G. and M. Gioffr{\`e} and R. Puglisi and D. Balduzzi and A. Galli and P. Ferraro",

year = "2011",

month = jun,

day = "14",

doi = "10.1117/12.889972",

language = "English",

isbn = "9780819486868",

series = "Proceedings of SPIE",

publisher = "SPIE",

editor = "Lin, {Charles P.} and Vasilis Ntziachristos",

booktitle = "Molecular Imaging III",

note = "European Conference on Biomedical Optics, ECBO 2011 ; Conference date: 22-05-2011 Through 26-05-2011",

}

Miccio, L, Finizio, A, Memmolo, P, Paturzo, M, Merola, F, Coppola, G, Di Caprio, G, Gioffrè, M, Puglisi, R, Balduzzi, D, Galli, A & Ferraro, P 2011, Detection and visualization improvement of spermatozoa cells by digital holography. in CP Lin & V Ntziachristos (eds), Molecular Imaging III., 80890C, Proceedings of SPIE, vol. 8089, Bellingham, Washington, European Conference on Biomedical Optics, ECBO 2011, Munich, Germany, 22/05/11. https://doi.org/10.1117/12.889972

TY - GEN

T1 - Detection and visualization improvement of spermatozoa cells by digital holography

AU - Miccio, L.

AU - Finizio, A.

AU - Memmolo, P.

AU - Paturzo, M.

AU - Merola, F.

AU - Coppola, G.

AU - Di Caprio, G.

AU - Gioffrè, M.

AU - Puglisi, R.

AU - Balduzzi, D.

AU - Galli, A.

AU - Ferraro, P.

PY - 2011/6/14

Y1 - 2011/6/14

N2 - Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it's able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.

AB - Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it's able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.

KW - digital holography

KW - image enhancement

KW - interference microscopy

U2 - 10.1117/12.889972

DO - 10.1117/12.889972

M3 - Conference contribution book

AN - SCOPUS:79960866785

SN - 9780819486868

T3 - Proceedings of SPIE

BT - Molecular Imaging III

A2 - Lin, Charles P.

A2 - Ntziachristos, Vasilis

CY - Bellingham, Washington

T2 - European Conference on Biomedical Optics, ECBO 2011

Y2 - 22 May 2011 through 26 May 2011

ER -

Detection and visualization improvement of spermatozoa cells by digital holography

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Keywords

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